Cell Signaling Technology
Phospho-Akt antibody
4060
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This product was used to test the activation of Akt in cancer cells in response to growth factors. 30 ugs of protein samples were used for this western blot to detect phospho Akt in PC3 cell lysates. I observed significant activation of Akt upon EGF treatment in PC3 cells.
Western Blot
Cancer cells
1:3000, overnight at 4 C
5% milk
1:3000, one hour at room temperature
NA
ECL and film
Upon treatment of PC3 cells with EGF, we observed activation of Akt as evidenced by increased band intensity at molecular weight ~60 kD.
None
Very good antibody for western blot
Good antibody for detecting phosphorylated Akt at S473 through western blotting.