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Date Published
Detection of Mouse Anti OVA Specific IgG2b Antibody
(1)
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BioLegend
November 25, 2015
To test antibody responses of TRPM2 -/- mice, wild-type (Trpm2+/+) or Trpm2-/- mice were subcutaneously immunized on day 0 and day 14 with ...
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CD4 Pacific Blue Antibody Review
(1)
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BioLegend
November 25, 2015
Spleens from latently infected mice are collected and processed for flow. Identification and quantification of various immune cells are then...
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Great Human Hif1a Antibody
(1)
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Cell Signaling Technology
November 24, 2015
Accurate detection of human Hif1a protein in cell lysates is crucial to our research, and this antibody has continued to perform well for ...
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CCR7 Expression in Human Primary PBMCs
(1)
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BioLegend
November 24, 2015
In this experiment, we investigated special subtypes of T-cells, mainly the T memory cell subsets, where CCR7 expression represents an ...
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TRI Reagent® for Nucleic acid isolation
(1)
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November 24, 2015
Total RNA was isolated from rat tissue (liver) using TRI Reagent®
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Double Immunofluorescence Staning
(1)
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Thermo Fisher Scientific
November 24, 2015
Hoechst is a nucleic acid stain which facilitates cell permeant nuclear counterstain and emits blue fluorescence when it bounds to the dsDNA...
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Utilization of CD45.1 as a Marker of Donor Leukocytes in Recipient CD45.2 Mice
(1)
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November 24, 2015
CD45 is common antigen expressed in all leukocytes. It has two different alleles, CD45.1 and CD45.2, which are functionally identical. We ...
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Detection of Mouse Anti-OVA Specific IgG1 Serum Antibody
(1)
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BioLegend
November 24, 2015
To test antibody responses of TRPM2 -/- mice, wild-type (Trpm2+/+) or Trpm2-/- mice were subcutaneously immunized on day 0 and day 14 with ...
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Sirt6 Expression in Neuronal Cells
(1)
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Abcam
November 24, 2015
This product was used to check Sirt6 expression levels in neuronal cells in different experimental conditions. The product is standardised ...
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Bcl-2 in Human T cells
(1)
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November 24, 2015
The aim of the research was to determine Bcl-2 expression in different human T cell subsets.
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Immunohistochemistry with SC48 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
November 23, 2015
I used this product to stain for and detect changes in delta fosb expression in rat brain nuclei associated with reward and memory following...
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Decent Antibody to Work With
(1)
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Cell Signaling Technology
November 23, 2015
PTEN is a tumor suppressor gene and has crucial role in MAPK signaling and apoptotic pathway. I used PTEN antibody from Cell signaling and ...
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PDPN Antibody Used for Western Blot in Human Podocyte Lysates
(1)
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November 23, 2015
In this experiment we performed Western blot analyses of PDPN protein expression in cell lysates of human Podocytes using the Anti-...
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Fast and Easy Plasmid DNA Purification Kit
(1)
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November 23, 2015
We used to use Qiagen HiSpeed Plasmid Midi Kit for Plasmid purification. It took a long time for the whole process. Zymoreseach's ZymoPure ...
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LI-COR Secondary Antibodies
(1)
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LICORbio
November 23, 2015
Following overnight incubation with primary rabbit antibodies, we selected this secondary anti-rabbit antibody to be able to detect specific...
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Perfect Nuclear Stain for Immunofluorescence Studies
(1)
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November 23, 2015
I often use immunofluorescence assay to determine protein localization in the cell. Generally, protein of my interest reside in the ...
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Anti-Mouse CD4 PE for Splenocyte Staining
(1)
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BioLegend
November 23, 2015
This antibody was used to identify CD4 T cells in mouse spleen in order to analyze overall cell numbers and subset differentiation under ...
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Quantification of TNFa by ELISA
(1)
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BioLegend
November 23, 2015
To analyze macrophage activation, THP1 cells were differentiated into macrophage cells and treated with fraction 10 or a control overnight. ...
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Portable Centrifuge Machine
(1)
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Eppendorf
November 23, 2015
Blood samples collected in vaccutainers were placed in 1.5 ml eppendorfs and centrifuged for plasma separation and also for protein and DNA ...
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Portable Pipet-Aid
(1)
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November 20, 2015
Pipet-aid was used for the transfer of sterile MRS media in test tubes for the culturing of lactic acid bacteria in laminar flow.
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Test of PLK2 in S1 and 293 Cells
(1)
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BosterBio
November 20, 2015
I wanted to investigate the expression of PLK2 protein after ShRNA-Plk2 treatment in UT7/Epo-S1 cell. Western blot was applied to test the ...
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Good SPARC Flow Antibody
(1)
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R&D Systems, a Bio-Techne Brand
November 20, 2015
Antibody worked fantastically with isolated murine central nervous system cells, identifying glial cells expressing SPARC with a high enough...
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Zeiss Axio Imager 2 Fluorescence Microscope with Apotome
(1)
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Carl Zeiss Microscopy
November 20, 2015
Zeiss AxioImager M2 with the apotome system is utilized for fluorescence and bright field Imaging. The use of the Apotome creates optical ...
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Good ELISA Assay for Mouse TSLP
(1)
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November 20, 2015
I study release of various cytokines by immune cells in response to antigens in vitro as well as in vivo.
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Good Staining to Identify CD45+ Cells in Human Peripheral Blood
(1)
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BD Biosciences
November 20, 2015
The aim of the research was a phenotypic analysis of human peripheral blood trying to use minimal amounts of sample. First, CD45+ cells ...
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Protein G Magnetic Beads
(1)
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New England Biolabs
November 20, 2015
These are good beads to be used for immunoprecipitations. They bind a variety of IgGs in different species and are compatible with the ...
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Excellent Caspase 3 Antibody
(1)
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Cell Signaling Technology
November 20, 2015
I check apoptosis induction in 293T cells by western blotting. This antibody works perfectly with no background, highly specific and ...
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"Scratch Assay" Using Ibidi Culture Inserts
(1)
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Ibidi GmbH
November 20, 2015
For migrational studies od adherent cells, it is necessary to introduce a scratch into a confluent or subconfluent cell culture. We found ...
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IRDye 680CT Goat Anti-Rabbit Antibody
(1)
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LICORbio
November 19, 2015
This antibody could be used in a variety of applications. We use it a lot in western blot together with the Li-cor Oddysey machine. Dilution...
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FoxO3 Antibody NBP2-16521 from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
November 19, 2015
This antibody was used to detect FoxO3 expression in different human head and neck cancer cell lines by western blot analysis. Two ...
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Easy to Use Real-Time PCR
(1)
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Bio-Rad
November 19, 2015
We use real-time PCR for gene expression studies. I selected the CFX Connect based on positive experiences with a similar touchscreen system...
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Western Blot Using Monoclonal Anti-Desmin Antibody
(1)
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November 19, 2015
In this experiment a a monoclonal Anti-Desmin antibody from Sigma Aldrich # D1033 was used (1:500) for Western blot.
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Best Kit for cDNA Synthesis
(1)
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Bio-Rad
November 19, 2015
I used Bio-Rad's cDNA synthesis kit for Reverse-transcriptase PCR reaction. I prepared 20 microlitres of cDNA for each sample and then ...
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FITC Anti-Mouse B220 Ab Used for Labeling Mouse B cells
(1)
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BioLegend
November 19, 2015
Our aim is to gate mouse B cell populations for downstream research. B220 is a common cell marker for B cells.
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CD103 Staining in Mouse Lung
(1)
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BioLegend
November 19, 2015
This antibody was used to stain mouse lung that has been processed to look for different population of DCs.
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Brilliant Violet Flow Antibody for CD25
(1)
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BioLegend
November 19, 2015
CD25 or IL-2 receptor alpha is only expressed on Treg cells and transiently expressed on activated T cells. I have never performed CD25 ...
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Very Good Kit for Intracellular Staining
(1)
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BD Biosciences
November 18, 2015
Splenocytes from mice were stimulated for 6 hrs with PMA and Ionomycin in the presence of protein transport inhibitor Golgi Stop. Cells were...
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Rocking Platform
(1)
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VWR International
November 18, 2015
This is a good everyday rocking platform to use with western blot incubations or general rocking of reagents.
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Assessing Total eIF2a
(1)
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Santa Cruz Biotechnology, Inc.
November 18, 2015
The purpose of this study was to assess a mechanism responsible for possible protein synthesis in a variety of treatments. Phosphorylated ...
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Perfect IL-33 ELISA Kit
(1)
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R&D Systems, a Bio-Techne Brand
November 18, 2015
I study release of various cytokines by immune cells in response to antigens in vitro as well as in vivo.
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Good Beta-Actin Antibody
(1)
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Cell Signaling Technology
November 18, 2015
I generally use beta-Actin for loading in western blotting. Cell Signaling's antibody is good but it has a long incubation time in contrast ...
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Labeling of CD4 Positive Cells in Human PBMC Samples
(1)
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BioLegend
November 18, 2015
This antibody gives quick staining, reliable results, great signal, and good value.
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High Quality Machine
(1)
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Roche
November 18, 2015
We use our MagNA Lyser to bead beat a variety of mouse of organs in order to collect RNA as well as create a supernatant to detect ...
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Santa Cruz Anti-Iba1 FL-147
(1)
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Santa Cruz Biotechnology, Inc.
November 18, 2015
The aim of my experiment was to stain for microglia following minocycline pretreatment. Minocycline suppresses the M1 activation of ...
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Migration and Wound Healing Assays
(1)
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Ibidi GmbH
November 18, 2015
Migration is an early event in atherosclerosis. Here we are analyzing cell migration behavior and cellular response on the molecular level ...
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Fluorescent Staining of Mouse Intestinal Epithelial Cells
(1)
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BioLegend
November 18, 2015
To detect mouse intestinal cells by IFA, intestinal tissue was collected from mice, then snap frozen and cryo sections were used for ...
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FACS Staining of RSV Infected PBMC
(1)
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November 17, 2015
This product was used to detect RSV infected PBMC by flow cytometry with single step staining.
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KAPA2G Fast HotStart PCR Mix is Great for Running Large Numbers of Genotyping PCRs
(1)
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Roche
November 17, 2015
Our laboratory utilizes a wide variety of mouse models, necessitating a large amount of genotyping. The KAPA2G PCR mix is great for this use...
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Detection of CD4 Positive T Cells in Mouse Breast Cancer Tumors
(1)
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BioLegend
November 17, 2015
The orthotopic syngeneic mouse tumors established using MVT1 cell line were analyzed for the presence of CD4+/CD3+ T cells using Alexa Fluor...
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STR-Testing Cell Line Authentication via Genetica DNA Labs
(1)
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November 17, 2015
Cell line contamination runs rampant among cell culture research, and often goes unchecked. Prominent research journals now require STR ...
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