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Date Published
CD4 Microbeads for Isolation of 2 Week Old Mouse Splenocytes
(1)
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Miltenyi Biotec
December 04, 2015
We aim to co-inject bone marrow and CD4 splenocytes into P0 mice to evaluate GVHD response as compared to bone marrow alone. We selected ...
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Phospho-EGFR (Tyr845) Antibody from Cell Signaling
(1)
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Cell Signaling Technology
December 04, 2015
This antibody was used to detect the expression of phosphorylated EGFR at Tyr845 site in response to cetuximab treatment in HN5 human HNSCC ...
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CD16/32 FITC Antibody in Lung Tissue
(1)
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BioLegend
December 04, 2015
CD16/32 was used as a marker for endothelial cells in a CD45 negative population in the lung homogenate.
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Nice RT-PCR Kit
(1)
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New England Biolabs
December 04, 2015
I amplified GAPDH as a loading control for 293T cells using NEB one tag RT-PCR kit. It works pretty well for mRNA analysis.
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Specific Secondary Antibody Reacts With Fab but Does Not React With Fc
(1)
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Abcam
December 04, 2015
This antibody is specific to Fab; it does not react with Fc. This was tested in western blotting loaded with human IgG and human Fc-fusion ...
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Western Blot with DNCIC1 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
December 04, 2015
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the ...
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Good Beta-Actin Antibody
(1)
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LICORbio
December 04, 2015
This beta actin antibody is sold by Li-Cor and works well using their Odyssey imaging system as a loading control.
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Good Microplate Reader
(1)
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Molecular Devices
December 04, 2015
The activity of enzymes that catalyze polymerization of G-actin to F-actin was estimated using fluorescent labeled G-actin. Samples ...
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Permafluor Mountant Is a Great Aqueous Mounting Medium
(1)
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December 04, 2015
After staining sections via immunofluorescence, the slides need to be mounted before visualization by microscopy.
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Good Ready Made Staining Solution
(1)
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Bio-Rad
December 03, 2015
Coomassie brilliant blue solution is required for staining the proteins bands after SDS-PAGE. I use the readymade solution from Bio-Rad and...
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Human LKB1 Antibody
(1)
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Cell Signaling Technology
December 03, 2015
Our lab works with a number of kinases, including LKB1/STK11. We recently transfected LKB1 deficient cells with an expression vector for...
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APC Anti-Mouse/Human IL-5 Antibody
(1)
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BioLegend
December 03, 2015
The purpose of this study was to identify IL-5 producing lymphocytes in the mediastinal lymph node after allergen sensitization in mice. The...
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Anti-Mouse Ly6G for Neutrophil Staining
(1)
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BioLegend
December 03, 2015
The general aim of my research is to look at how inflammatory response can be modulated by lipid molecules. I wanted to examine the number ...
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FITC Anti-Mouse CD3e Ab Used for Labeling Mouse T-cells
(1)
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BioLegend
December 03, 2015
Our aim is to gate T cell populations for downstream research. CD3e is a common cell marker for T cells.
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Best Phosphatase Inhibitor Cocktail
(1)
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MilliporeSigma
December 03, 2015
Our lab works with cell lysates to study various proteins related to cancer. Many of these proteins are quickly degraded by phosphatases, ...
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Fantastic Thermal Cycler
(1)
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Thermo Fisher Scientific
December 03, 2015
We use our Thermal Cyclers for genotyping PCR reactions and LDPCR. Most protocols run between 2-3 hours.
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Nkx6.1 Antibody Staining in Mouse Pancreatic Tissue
(1)
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December 03, 2015
Nkx6.1 is an essential islet beta cell specific transcription factor. Nkx6.1 maintains normal islet beta cell function and fates. This ...
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Evaluation of Reactive Oxygen Species (ROS) Generation in Cells Using DCFDA
(1)
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Thermo Fisher Scientific
December 03, 2015
Cellular ROS levels can be measured in live cells by a technique that converts 2’,7’ –dichlorofluorescin diacetate (DCFDA) which is oxidized...
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IgD FITC Antibody Review
(1)
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BioLegend
December 02, 2015
Splenocytes from latently infected mice are collected at various time points. Their immune cell populations are identified and quantified to...
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Excellent Antibody for Staining CD4+ T Cells
(1)
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BioLegend
December 02, 2015
Our laboratory is very interested in the role of regulatory T cells in autoimmunity. We mainly focus on regulatory CD4+FoxP3+ T cells and ...
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Great CD11b Antibody for Flow Cytometry
(1)
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BioLegend
December 02, 2015
This antibody was used in a study designed to analyze the recruitment of stromal cells to the tumor in breast cancer mouse models. The goal ...
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Best PCR Master Mix Available
(1)
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Bioline
December 02, 2015
Our lab conducts a great deal of translational research with transgenic mice, and we run a variety of PCR's to genotype mice. We rely on...
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p-eIF2alpha
(1)
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Novus Biologicals, a Bio-Techne Brand
December 02, 2015
The general aim of this project was to assess total protein translation attentuation in a variety of treatment groups using total and ...
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Super Specific Secondary Antibody Against Mouse IgG1 Heavy Chain Only
(1)
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Abcam
December 02, 2015
This antibody is super specific! It does not react with mouse IgG2a or human IgG, or any IgG light chain I have tested so far. I use it ...
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Time-Dependent Measurement of Intracellular Calcium Levels in Live Cells
(1)
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Thermo Fisher Scientific
December 02, 2015
Intracellular calcium levels are key indicators of any signaling system involving Ca2+ as second messenger. Stimulation of cells is involved...
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Sirt7 Expression in Neuronal Cells
(1)
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Abcam
December 02, 2015
This product was used to check Sirt7 expression in neuronal cells. The product is standardised for western blot.
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Separation of Epidermis from Dermis Using Dispase Solution (Matrix Metalloprotease)
(1)
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Corning Life Sciences
December 02, 2015
Isolation and culture of keratinocytes from human epidermis is possible starting from a section of tissue, punch biopsies or foreskin ...
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Great Human Hif2a Antibody
(1)
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Cell Signaling Technology
December 01, 2015
This is arguably the best antibody for human Hif2a that I have found on the market. We have been using it for years in lab, and have no ...
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Expression of Ectonucleotidase CD39 on Th1 Cells
(1)
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BioLegend
December 01, 2015
A chronic state of inflammation as in the case of sepsis can cause Th1 cells to self regulate the inflammatory condition. We wanted to see ...
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Excellent a2-Macroglobulin ELISA Kit
(1)
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December 01, 2015
We needed a protein assay to measure a2-macroglobulin in nasal secretions, which is a biomarker for epithelial leak. This kit was easy to ...
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Human CRGP ELISA Kit that Works
(1)
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Cayman Chemical
December 01, 2015
This CRGP enzyme immunometric assay kit is based on a double-antibody sandwich technique. It comes with a pre-coated 96-well plate and ...
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Vortexer
(1)
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VWR Scientific Products
December 01, 2015
This is a good machine for vortexing samples. The base allows for a sturdy base to put your samples so they don't slip. Also has adjustable ...
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Refrigerated Table Top Centrifuge
(1)
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December 01, 2015
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti-cancer immune therapies.
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CD3 PE Antibody Review
(1)
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BioLegend
December 01, 2015
Splenocytes from latently infected mice are analyzed for various immune cells (T and B cells) and those are quantified at various time ...
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Anti Platelet Antibody - Isotype
(1)
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Abcam
December 01, 2015
This isotope control is used in our lab for anti platelet antibody test, where we use a monoclonal to detect the presence of bound IgG to ...
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HIF-1alpha Antibody from BD Transduction Laboratories
(1)
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BD Biosciences
December 01, 2015
This antibody was used to detect the expression of the alpha subunit of HIF-1 in response to cetuximab treatment in human HNSCC cells under ...
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Not Specific to LXR-Beta
(1)
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Abcam
December 01, 2015
This antibody recognizes both LXR alpha and LXR beta.
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Antibody Recognizes Both Isoforms
(1)
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Abcam
November 30, 2015
The antibody was generated to recognize LXR alpha, however is also recognizes LXR beta. This was verified with cells from KO animals.
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Differentiation of Stem Cells
(1)
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November 30, 2015
I wanted to use BMP4 as a positive control for my stem cells since I was trying to identify new compounds that can induce differentiation in...
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Mini BioMixer With Dimpled Mat
(1)
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November 30, 2015
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti-cancer immune therapies.
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Cytoplasmic and Nuclear Protein Extraction
(1)
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November 30, 2015
This Kit is used to prepare the cytoplasmic and nuclear extracts from cultured cells and also in tissue. We can prepare active non-denatured...
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Highly Sensitive Metabolic Assay Equipment
(1)
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Agilent Technologies
November 30, 2015
Our lab conducts research on a number of cancers, and our research often includes an analysis of the metabolic effects of various mutations,...
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Click-It Metabolic Labeling
(1)
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Thermo Fisher Scientific
November 30, 2015
The aim of this project was to detect total cellular protein synthesis in a variety of treatments using click-it chemistry. We selected this...
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CD5-APC Antibody Review
(1)
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BioLegend
November 30, 2015
Splenocytes from latently infected mice are collected, processed, and stained for various immune cell markers to identify different ...
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Nice Orbital Shaker
(1)
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November 25, 2015
This is a good orbital shaker for growing bacteria for cloning or protein expression. It is a large shaker capable of shaking multiple large...
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Competitive Substance P ELISA Kit
(1)
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Cayman Chemical
November 25, 2015
This ELISA kit assay is based on the competition between substance P and a AchE conjugate for limited amount of substance P- specific rabbit...
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Excellent Master Mix for Quantitative RT-PCR
(1)
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November 25, 2015
I transfected 293T cells to knock down AKT by transient transfection. After 72 hours, I checked the knock down by quantifying the mRNA using...
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Macrophage-Like Cells in the Small Intestine Stain for CD16/32
(1)
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BD Biosciences
November 25, 2015
I wanted to look at whether expression of CD16/32 on myeloid cells in the small intestine appeared to differ in a healthy vs diseased mouse.
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Perfect Cytospin Cytocentrifuge
(1)
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November 25, 2015
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti-cancer immune therapies.
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Anti-PDL-1 Antibody Treatment Reduces Expression of the Exhaustion Marker PD-1 in T Cells
(1)
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BioLegend
November 25, 2015
Elevated expression of the exhaustion marker PD-1 is a major cause of programmed cell death of activated T cells. We hypothesized blocking ...
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