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Western Blot Using Anti-Akt1/PKBa (#07-416)
(1)
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MilliporeSigma
December 29, 2015
Here, we performed Western blot analyses of Akt1/PKBa protein expression in cell lysates of human podocytes using the Akt1/PKBa antibody ...
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Good Fluorescent GAPDH Antibody
(1)
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Abcam
December 29, 2015
I am doing numerous western blots on cell lysates and I always use this antibody as a GAPDH loading control.
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Best Compact Thermocycler on the Market
(1)
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Thermo Fisher Scientific
December 29, 2015
Our lab works with mice, which involves a great deal of genotyping. We run a number of different PCR's for to detect the presence of various...
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PE/Dazzle™ 594 anti-mouse/human CD11b
(1)
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BioLegend
December 29, 2015
Mouse lungs were extracted and digested with collagenase and DNase. Cells were then stimulated with PMA + ionomycin. Cells were stained ...
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Excellent Gel Dryer
(1)
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Hoefer, Inc.
December 29, 2015
Stained polyacrylamide gels used to resolve proteins were dried in GD2000 Vacuum Gel-Drying System either for preservation or for downstream...
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Clear Staining for Peritoneal Macrophages
(1)
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BioLegend
December 29, 2015
Our work involves the characterization of mouse macrophages from the peritoneal cavity. In order to gate most effectively the peritoneal ...
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T Cell Marker in Human Tissue
(1)
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BioLegend
December 28, 2015
This antibody was used to identify the CD3 population in human tissue.
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Alpha Tubulin for Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
December 28, 2015
This was an experimental project to test whether we could visualize alpha tubulin under our fluorescent microscope.
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Best Infrared Sterilizer for Hood Use
(1)
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December 28, 2015
Maintaining a sterile work environment in the hood can be challenging, especially when it comes to aspirating off liquids from plates. ...
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An Excellent Centrifuge Machine
(1)
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Eppendorf
December 28, 2015
I used this centrifuge machine for centrifugation of various biological samples and isolation of RNA, DNA and plasmid. This machine provides...
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Western Blot Using Anti CPXM2 from Acris Antibodies GmbH
(1)
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December 28, 2015
We performed Western blot analyses of CPXM2 protein expression in cell lysates of HEK293 cells using the anti CPXM2 antibody from Acris ...
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Western Blot Analysis of HIF1 Alpha
(1)
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Novus Biologicals, a Bio-Techne Brand
December 28, 2015
This antibody was used to detect HIF1a in PC3 cells through western blot analysis. This antibody was purchased from novus biologicals and ...
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Good Performance of Microprocessor Based Hot Plate With Magnetic Stirrer
(1)
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Corning Life Sciences
December 28, 2015
Submerged western transferred unit was placed in Corning magnetic stirrer, with a rotation value of around 800rpm. Proteins were western ...
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Excellent Alternative to Traditional Western Blotting
(1)
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LICORbio
December 28, 2015
This system is used in conjunction with IR antibodies to perform western blots. This system allows to probe for multiple proteins at once ...
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Great TNF alpha Kit
(1)
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BosterBio
December 28, 2015
This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product.
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Canine Immunophenotyping Antibodies
(1)
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Bio-Rad
December 23, 2015
High expression of MHCII is associated with T cell lymphomas. This Antibody is used to detect expression of MHC II on monocytes and ...
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Best Electronic Multichannel Pipette
(1)
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Eppendorf
December 23, 2015
Repeatedly pipetting with multichannel pipettes can be extremely exhausting for your forearms, and quite tedious for a set of 96-well plates...
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Immunofluorescence with DNCIC1 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
December 23, 2015
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the ...
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Efficient Ligation of DNA Fragments for Cloning
(1)
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December 23, 2015
This assay kit was used for joining DNA fragments into a cloning vector.
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Western Blot Using Anti-Angiotensin II Type 1 Receptor Antibody #ab18801
(1)
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Abcam
December 23, 2015
We performed Western blot analyses of AT-1R protein expression in cell lysates of human podocytes using the anti-Angiotensin II Type 1 ...
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Good System for Fast and Efficient Concentration of Liquid Samples
(1)
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December 23, 2015
Commercially ordered siRNAs were packed in invivofectamine reagent (transfection reagent) using standard protocol. One hundred microliter of...
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Pierce BCA Total Protein Quantitation Kit
(1)
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December 23, 2015
This BCA protein assay kit is a detergent-compatible formulation based on bicinchoninic acid for the colorimetric detection and ...
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Licor Donkey Anti-Rabbit 800CW Antibody
(1)
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LICORbio
December 23, 2015
This secondary antibody was used to detect rabbit primary antibodies. This was used in the Li-Cor Odyssey imaging system. It is a good ...
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GFP Antibody for Immunoprecipitation
(1)
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Thermo Fisher Scientific
December 23, 2015
Here, GFP antibody from Thermo Fisher was tested for immunoprecipitation of a GFP tag expressed in HEK293T cells.
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Easy to Use DNase Kit
(1)
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QIAGEN - Sample to Insight
December 22, 2015
This small kit comes with DNase enzyme tube and two buffer tubes. It is easy to make working solution and only need 15 min at room ...
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Anti-mouse CD103 APC for Splenocyte Staining
(1)
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BioLegend
December 22, 2015
Splenocytes were analyzed for the presence of CD103+ DC under various conditions (eg tumor bearing vs non tumor bearing mice)
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Antibody is Non-Specific
(1)
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Novus Biologicals, a Bio-Techne Brand
December 22, 2015
We used this antibody to screen cdyl2 knockout candidates using Western Blots. The first lane shows wildtype cell lysate while the rest show...
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Western Blot Using Anti-Nephrin Antibody from Abcam
(1)
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Abcam
December 22, 2015
We performed Western blot analyses of nephrin protein expression in cell lysates of human podocytes using the anti-Nephrin antibody from ...
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Western Blot Analysis of HA Tag
(1)
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GeneTex
December 22, 2015
This antibody was used to detect HA tag of target protein expressed in HEK293T cells through western blot analysis. This antibody was ...
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Good Surface Staining for CD56+ Human Peripheral Blood Cells
(1)
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Beckman Coulter Life Sciences
December 22, 2015
The aim of this research was a phenotypic analysis of human peripheral blood cells to identify major leukocyte subsets using minimal ...
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HA Antibody for Western Blotting
(1)
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BioLegend
December 22, 2015
This antibody was used to test the expression of a HA tagged protein overexpressed in HEK293T cells. The end goal of this experiment was to ...
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Perfect Total AKT Antibody
(1)
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Cell Signaling Technology
December 22, 2015
I am investigating the role of the AKT-Pathway after extracellular vesicle treatment. I chose this product since I am also using the total ...
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Anti-FXR1 in Western Blot
(1)
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December 22, 2015
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the ...
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Best Phosphatase Inhibitor Cocktail on the Market
(1)
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MilliporeSigma
December 22, 2015
Our lab works with cell lysates to study various proteins related to cancer. Many of these proteins are quickly degraded by phosphatases, ...
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Western Blot Using Caspase-3 (3G2) Mouse mAb from Cell Signaling Technology
(1)
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Cell Signaling Technology
December 21, 2015
With this antibody, we performed Western blot analyses of Caspase-3 in cell lysates of human podocytes using the Caspase-3 (3G2) mouse mAb ...
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Antibody Gives Good Signal and Specific Staining
(1)
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BioLegend
December 21, 2015
Mouse bone marrow cells were stained for the population of granulocytes in specific knock out mice.
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Iba1 in Rat Brain
(1)
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December 21, 2015
Anti Iba1 antibody from Wako was used to label microglia in rat brain areas associated with reward and memory such as hippocampus, nucleus ...
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Fast Transfer System
(1)
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Bio-Rad
December 21, 2015
This system is used for protein transfers for western blots. The system is easy to use and when using TGX mini gels, protein transfer is ...
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Flow Cytometry Antibody with Good Signal but Some Leakage
(1)
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BioLegend
December 21, 2015
CD11c distribution was used to study osteoclast precursor subsets in the spleen and bone marrow.
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Quality GAPDH Antibody
(1)
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GeneTex
December 21, 2015
Our lab aims to elucidate the role of kinases in several cancers, and we are often performing Western Blots to determine kinase protein and ...
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Excellent Refrigerated Micro Centrifuge for Research Purposes
(1)
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December 21, 2015
Whole cell extract was prepared from mouse cell lines. For this, growth factor treated or untreated cells were lysed in buffer and crude ...
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Great Mutation Detection Kits
(1)
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MyBioSource.com
December 21, 2015
We have used this kit for our CRISPR mutation detection many times. It always give us great results. It is reliable.
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Western Blot Analyses Using Anti-Phospho-mTOR (Ser2448)
(1)
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MilliporeSigma
December 18, 2015
Here we performed Western blot analyses of phospho-mTOR in cell lysates of human podocytes using the anti-phospho-mTOR (Ser2448) antibody.
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Anti-GFP for DAB Stain
(1)
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Thermo Fisher Scientific
December 18, 2015
The aim of the research was to incorporate light activated proton pumps into the membrane of new neurons and to be able to inactivate the ...
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Great Electrophoresis System
(1)
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December 18, 2015
We use our gel system to run gels for genotyping and LDPCR. They are used continuously every day. Use Agarose gel and TAE buffer. 40-100 mV ...
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cDNA Synthesis Kit
(1)
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December 18, 2015
This kit is used to transform RNA into cDNA. This kit contains a random primer mix and the RT is not already into the buffer. This means one...
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Il-1 beta Antibody from Novus
(1)
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Novus Biologicals, a Bio-Techne Brand
December 18, 2015
I am using the NB600-633 antibody to stain for cytokines following microglia M1 suppression using Minocycline 30mg/kg in rat brain paraffin ...
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Santa Cruz Biotechnology GR Antibody (H-300)
(1)
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Santa Cruz Biotechnology, Inc.
December 18, 2015
I bought this antibody to measure GR expression in rat brain. The specific goal was to quantify the number of GR+ neurons in the rat ...
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A Cheap Primary Antibody With OK Protein Recognization
(1)
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MilliporeSigma
December 18, 2015
This is a cheap primary antibody compared with all other common primary antibodies from rabbit. Dilution is also okay, 1:2000 does the job. ...
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Very Good Staining to Identify CD3+ Cells
(1)
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December 18, 2015
The aim of this research project was to immune phenotype lymphocyte subsets in human peripheral blood using minimal amount of samples.
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