The Developmental Studies Hybridoma Bank
Nkx6.1 Antibody
F64A6B4
Browse Similar Products: Nkx6.1 Immunofluorescence Antibodies
Nkx6.1 is an essential islet beta cell specific transcription factor. Nkx6.1 maintains normal islet beta cell function and fates. This regulator has been found to be compromised in Type 2 diabetic islet beta cells. We have focused on establishing the expression pattern of Nkx6.1 in Type 1 diabetes. We have immunostained normal mouse pancreas slides to determine the right concentration to use for detection by immunofluorescence.
Immunofluorescence
Mouse pancreatic cryo-section
1:1000, 4 Deg C, overnight.
5% Normal Donkey Serum in 1%BSA/PBS (w/v)
1:1000. mouse IgG-Cy3. 2 hours, Room temperature
NA
Fluorescence microscopy
Nkx6.1 staining was specific for islet beta cells in 8 week old mouse pancreas tissue. Insulin (green) and Glucagon (white) were utilized for determining specific binding in islet beta cells, and not alpha cells.
None
Good detection, low background at 1:1000
This antibody works great for Nkx6.1 detection by immunofluorescence. It is offered at a price equal to production costs, which is ~8-10 times less than commercial distributors.