An Excellent Alternative to the Mouse anti-NeuN Antibodies

Molecular Physiology and Biophysics
Baylor College of Medicine
Other

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Company:

EMD Millipore

Product Name:

Anti-NeuN (rabbit) Antibody

Catalog Number:

ABN78

Info: View Product View Product Specs

Browse Similar Products: NeuN Antibodies

This antibody works very well and provides a good signal to noise ratio. Its pattern of staining is equivalent to the monoclonal mouse anti NeuN antibodies that our lab has used for immunofluorescent histology. For immunofluorescent histology, this antibody works well as a general staining marker for neuronal cells. Although not every neuron will express NeuN, most of the neurons in our brain regions of interest do (mouse brainstem neurons). Adding this antibody to our collection has given us more flexibility for co-staining combinations where use of two different mouse antibodies at the same time was impractical. 

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Paraformaldehyde fixed mouse and rat brain.

Primary Incubation

1:500 dilution in blocking buffer overnight at 4C

Blocking Agent

10% goat serum in 1xPBS, 0.3% tritonX100 (2hrs at RT)

Secondary Incubation

1:500 of JaxIR fluorescent conjugated anti rabbit AB, diluted 1:500 into blocking buffer (4hrs at RT)

Tertiary Incubation

NA

Detection

Fluorescence

Results Summary

We were able to generate several images using NeuN as a neuronal marker to qualitatively and quantitatively score colocalization and expression of other proteins within neurons. The attached image shows staining with the Rabbit NeuN antibody within Rat brain (we obtain similar staining results with mouse tissue as well).

Additional Notes

None

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Summary

The Good

Great signal to noise

The Bad

A bit pricier than the mouse monoclonal against NeuN

The Bottom Line

It marks neurons, and it is raised in Rabbit. It is a good antibody to have available for immunostaining mouse brain (it also works with Rat brain)

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