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Date Published
Good Cytochrome C Antibody
(1)
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Cell Signaling Technology
November 05, 2015
I used Cytochrome C antibody to check apoptosis upon treatment in 293T cells. This antibody gave specific bands but requires long exposure.
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Good Antibody for Identifying Macrophages
(1)
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BioLegend
November 04, 2015
Latently infected mouse PEC's were collected and stained, and CD11b+ cells were sorted out as macrophages from other immune cells.
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Western Blot Densitometry in LI-COR Image Studio
(1)
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LICORbio
November 04, 2015
Analyzing western blots is necessary to measure and quantify changes due to numerous treatment conditions.
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Expression of Activation Marker F4/80 on Macrophage Cells Isolated from Mouse Spleen
(1)
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BioLegend
November 04, 2015
Macrophages expressing F4/80 and CD11b+ are considered to be in an activated stage. We artificially induced ischemic stroke in male and ...
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Very Good and Convenient Micro Centrifuge
(1)
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Eppendorf
November 04, 2015
This centrifuge is a compact style and only takes up a little space. It is very reliable and runs quietly even at high speed such as ten ...
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Transfection Using Fugene HD
(1)
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Promega
November 04, 2015
This product was used for transfection of JEG3 cell lines.
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Very Poor Results, Quite Expensive Product
(1)
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Abcam
November 04, 2015
This antibody was used in whole liver tissue and subcellular fraction samples to measure presence of the mitochondrial associated membrane (...
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Nod1 Expression in Mouse T Cells
(1)
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Cell Signaling Technology
November 04, 2015
The aim of the research was to determine the levels of Nod1 in differentiated mouse Th1 and Th17 cells.
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Anti-Rat Ig Compensation Beads
(1)
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BD Biosciences
November 04, 2015
We have used these beads for flow cytometry to optimize the fluorescence compensation settings in the multicolor flow cytometric analyses.
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Good Anti-FoxP3 Antibody for Nuclear Staining
(1)
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November 04, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Highly Specific Antibody
(1)
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November 03, 2015
I stained mouse thymocytes for Cd4 + cells using CD4-FITC antibody. I stained for 20 minutes on ice and got perfect staining for my samples.
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Anti-GFP to Study Protein Co-Localization from Cytoplasm to Nucleus
(1)
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November 03, 2015
This product was helpful in an experiment to visualize the localization of my protein of interest from cytoplasm to nucleus.
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Staining of Neutrophil Cells by FACS
(1)
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BioLegend
November 03, 2015
To analyze neutrophil recruitment, mice were pretreated with PBS or a drug (LF fraction 10). 48 hours later, mice were injected with LPS via...
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Best p38MAPAK Ab for Immunodetection
(1)
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Cell Signaling Technology
November 03, 2015
Cells were treated and proteins were extracted to check the normal p38MAPK levels by western blotting. Equal amounts of proteins were ...
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Good Sample Rocker
(1)
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November 03, 2015
Use this to rock a variety of samples. Large platform allow for rocking of everything from tubes to boxes. Can use for a variety of ...
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CO2 Cell Culture Incubator From Eppendorf
(1)
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Eppendorf
November 03, 2015
This incubator works well in the lab and the LED screen shows the parameter information well. It is easy to move around compared to the old ...
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Great Antibody for Intracellular Staining
(1)
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BioLegend
November 03, 2015
This antibody was recommended by a collaborating lab for the detection of IFNg-producing CD8+ T cells after restimulation with non-specific ...
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Lucifersae Assay
(1)
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Promega
November 03, 2015
Reporter systems are generally used to study eukaryotic gene expression. These dual reporters are generally used for experimental accuracy. ...
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Anti-Cyclin A Antibody
(1)
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November 02, 2015
This monoclonal anti-Cyclin A antibody was used to study cell cycle regulating kinases in cancer.
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Sepharose G Beads for Immunoprecipitation Application
(1)
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November 02, 2015
These beads are used to enrich a protein of low abundance by the pull down method. Basically it binds according to the IgG, upon the source ...
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B220 PECy7 Antibody Review
(1)
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BioLegend
November 02, 2015
This antibody was used to identify and sort out specific B cell populations in latently infected mouse peritoneum.
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Good Flow Cytometry Antibody, Gives Nice Signal
(1)
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BioLegend
November 02, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-...
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Nice Akt Antibody
(1)
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Cell Signaling Technology
November 02, 2015
I check total endogenous AKt expression in two cell lines: 293T and BT474. The antibody worked fine but gave a non specific band at low ...
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Myeloid Lineage Marker Staining
(1)
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BioLegend
November 02, 2015
CD11b is expressed on several immune cells including macrophages and monocytes. I used this antibody for identifying CD11b high macrophages ...
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Expression of the MIF Receptor CD74 in Activated Macrophages and Microglial Cells After Ischemic Stroke
(1)
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November 02, 2015
It is well established that experimental stroke triggers inflammation in brain and activates the peripheral immune system with a rapid ...
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Very Good Cell Sorting Machine
(1)
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BD Biosciences
November 02, 2015
The objective of this experiment was to separate human CD45 expressing cells from mouse cells.
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Detection of Mouse Intestinal Cells
(1)
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Abcam
October 30, 2015
To analyze the activation of mouse intestinal macrophages after experimental treatment, mice were gavaged with reagent and intestinal tissue...
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Good Anti-CD4 Antibody for FACS
(1)
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October 30, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Good Transfection Reagent for Mammalian Cell Lines
(1)
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QIAGEN - Sample to Insight
October 30, 2015
The aim of our study was to transfect siRNA in mammalian cells to achieve siRNA knockdown at the protein level. Selection of HiPerFect ...
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Great cDNA Synthesis Kit
(1)
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QIAGEN - Sample to Insight
October 30, 2015
I used this kit to screen positive colonies of transformants of ESAT6. It has a genomic DNA removal step which helps to avoid genomic DNA ...
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Good PCNA Antibody
(1)
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Santa Cruz Biotechnology, Inc.
October 30, 2015
I used anti-PCNA antibody for immunohistochemistry. PCNA is a cell proliferation marker so, it shows nuclear localization in cancer tissue ...
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Rapid Genotyping of Rodents With Tail PCR
(1)
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Viagen
October 30, 2015
The aim of the experiment was to test the mouse genotype for Par1 allele. For this, mouse tail DNA was isolated using Direct PCR (Tail), PCR...
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Reliable and Easy-to-Use RNA Extraction Kit
(1)
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QIAGEN - Sample to Insight
October 30, 2015
This kit is used to extract RNA from up to 5X10^5 cells or less than 5mg tissue. It is very reliable and easy to use.
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Very Good Anti-mouse IFN-Gamma Antibody
(1)
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BD Biosciences
October 30, 2015
The Aim of this experiment was to standardize intra-cellular staining of IFN-gamma. Mouse spleen cells were stimulated (6 hours) with PMA ...
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Cell Lysis Buffer That Works
(1)
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Cell Signaling Technology
October 30, 2015
This cell lysis buffer is used to lyse cells under nondenaturing conditions. The lysis effect in relatively mild and we use it together with...
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Great Way to Isolate Monocytes from PBMCs
(1)
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Miltenyi Biotec
October 29, 2015
With this product we achieved successful isolation of monocytes from human PBMCs with >90% purity of CD14+ monocytes and functionally active...
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Caspase-3 Apoptosis Kit
(1)
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BD Biosciences
October 29, 2015
I used 293T cells to induce apoptosis by oxidative stress. I check apoptosis by determining the active caspase 3 using this kit. The reason,...
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xCT Antibody NB300-318 from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
October 29, 2015
This antibody was used to detect xCT expression in different breast cancer cell lines by western blot analysis. Two antibodies for xCT from ...
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Labeling of Human PBMCs with CD62L BV421 Antibody
(1)
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BioLegend
October 29, 2015
This CD62L antibody is included in a flow cytometry panel in order to identify L-Selectin expression on human PBMCs. Because multiple ...
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Cell Viability Stain
(1)
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BioLegend
October 29, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-...
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Good ELISA Kit for Human IL-8 Protein Detection
(1)
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Thermo Fisher Scientific
October 29, 2015
This kit includes coating antibody, detection antibody, standards and streptavidin-HRP reagents. Need to order buffers and solution ...
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Flow Cytometric Method of Estimation of Glucose Uptake Using 2NBDG
(1)
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Cayman Chemical
October 29, 2015
2NBDG, a fluorescent analogue of glucose which represents amount of glucose uptake by cells. Measuring the amount of fluorescence by a ...
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Ultra Low Temperature Freezer
(1)
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Eppendorf
October 29, 2015
This freezer is used to keep samples at -80 Celsius. The freezer has a large capacity and has not had issues with keeping temperature.
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GoBlot processor - Time saver!
(1)
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October 28, 2015
We have gotten four GoBlot processor machines so far Pros: 1. Time saving 2. Easy to handle 3. Convenient programs 4. Less buffer needed 5. ...
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Detection of Mouse Intestinal Macrophages by IFA
(1)
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BioLegend
October 28, 2015
To analyze the activation of mouse intestinal macrophages after experiment treatment, mice were gavaged with reagent and intestinal tissue ...
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Excellent Pre-Optimized Protein Estimation Kit
(1)
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October 28, 2015
Protein estimation is routine work that is generally performed prior to western blotting, SDS-PAGE etc. I use this kit for estimating the ...
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Labeling CD16 Positive Cells in Human PBMC Sample
(1)
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BioLegend
October 28, 2015
This is a good antibody for discriminating CD16 postive and negative cells such as monocytes, etc
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Bio-Rad Primers for RT-qPCR
(1)
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Bio-Rad
October 28, 2015
We are assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated by density ...
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Coverslip Mounting Solution With DAPI
(1)
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Thermo Fisher Scientific
October 27, 2015
This is a mounting solution for microscopy cell viewing. First of all, this mounting solution has DAPI in it, which brings up the price than...
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Lab Rotator
(1)
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Glas-Col, LLC
October 27, 2015
Rotation module with interchangable heads (additional costs). This system is easy to use and works well even in prolonged time in the cold ...
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