Product Reviews

As part of our interest in proteostasis, we have used this kit to measure proteasomal activity. Commercially, there seems to be two ... Read Review

This antibody was used in our study of proteasome inhibitor resistence in multiple myeloma published in Cancer Cell (Leung-Hagesteijn et al,... Read Review

In vitro cultured bone marrow derived cells were checked for the expression of CSF-1R under osteoclast differentiation conditions from mice. Read Review

We routinely use this antibody in our 8-10 color multi parameter flow cytometry analysis and the CD8a antibody conjugated to APC-eF780 works... Read Review

When a rabbit-derived primary antibody is used to stain an antigen of interest, an anti-rabbit secondary antibody is necessary to visualize ... Read Review

Though luminex kits can measure the concentration of multiple cytokines from within a serum sample, mouse IFN alpha is not within those kits... Read Review

We plan to identify colon cancer specific genetic mutations from patient plasma samples. DNA isolation was needed to prepare whole genome ... Read Review

Mammalian cells were cultured up to 70 percent confluence, serum starved for 12h and treated with growth factor. Proteins were extracted in ... Read Review

I used this EcoRV restriction enzyme along with BglII for the cloning of my gene of interest into a plasmid vector. Subsequently I used this... Read Review

This product was used to culture glioma stem cells from U87 cells. The product is a must to add when culturing cancer stem cells. Read Review

We use streptavidin-coated beads to capture various biotinylated targets. The magnetic beads are collected using neodymium magnet stands for... Read Review

U2OS cells transfected with HA-SceI were lysed in sample buffer and proteins were separated in 4-20% gradient SDS-PAGE. Ha-SceI expression ... Read Review

Testing GLUT 1 antibody as a marker for plasma membrane. Read Review

I am comparing the size of the endoplasmic reticulum to the size of the whole cell in a variety of treatment conditions. The ER was ... Read Review

I have used the Merck Millipore columns to concentrate Total RNA. I wanted to maximise RNA recovery by using a number of sample aliquots for... Read Review

I use this staining kit to measure T-cell proliferation induced by our stimuli. Since I have FITC conjugated antibodies in my staining ... Read Review

Cells were grown and treated with growth factor to test its effect on mRNA expression. RNA was extracted with conventional TriZol method. ... Read Review

I use this antibody for the characterization of human Treg cells based on CD+CD25+Cd127low criteria. Cd127 is an additional marker for ... Read Review

I use this kit in the lab to extract RNA from cells. The RNA Integrity is quite high and it is an easy kit to use. Read Review

We study the distribution of gamma delta cells in response to minicircle DNA injections. With such a small subset, a good flurochrome ... Read Review

For characterising antibody responses to SIV mac gp140 following vaccination or natural infection, ELISAs can be carried out to quantify ... Read Review

One day prior the experiment, isolate monocytes using Ficoll gradient centrifugation. Afterwards, use magnetic bead separation to extract ... Read Review

We utilize the REDExtract kit to quickly obtain DNA from mouse tails for genotyping. We looked into using this product because it was much ... Read Review

The HA tag is short, so a clean and specific antibody is essential. Sigma's anti-HA is generally a good antibody for western blot and ... Read Review

The aim of this experiment was to detect cytokines in mouse T cells. This antibody is good to distinguish the T cell population. Read Review