Anti-Mouse Ly6G for Neutrophil Staining

Medicine
Harvard Medical school
Post doctoral research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse Ly-6G

Catalog Number:

127615

The general aim of my research is to look at how inflammatory response can be modulated by lipid molecules. I wanted to examine the number of neutrophils in my samples therefore we purchased the Ly6G antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

BALF

Primary Incubation

1:200, 20 mins

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

The antibody clearly separate out the Ly6G positive population in the mouse BALF

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It works

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