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Date Published
Great cDNA Synthesis Kit
(1)
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QIAGEN - Sample to Insight
October 30, 2015
I used this kit to screen positive colonies of transformants of ESAT6. It has a genomic DNA removal step which helps to avoid genomic DNA ...
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Good PCNA Antibody
(1)
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Santa Cruz Biotechnology, Inc.
October 30, 2015
I used anti-PCNA antibody for immunohistochemistry. PCNA is a cell proliferation marker so, it shows nuclear localization in cancer tissue ...
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Rapid Genotyping of Rodents With Tail PCR
(1)
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Viagen
October 30, 2015
The aim of the experiment was to test the mouse genotype for Par1 allele. For this, mouse tail DNA was isolated using Direct PCR (Tail), PCR...
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Reliable and Easy-to-Use RNA Extraction Kit
(1)
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QIAGEN - Sample to Insight
October 30, 2015
This kit is used to extract RNA from up to 5X10^5 cells or less than 5mg tissue. It is very reliable and easy to use.
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Very Good Anti-mouse IFN-Gamma Antibody
(1)
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BD Biosciences
October 30, 2015
The Aim of this experiment was to standardize intra-cellular staining of IFN-gamma. Mouse spleen cells were stimulated (6 hours) with PMA ...
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Cell Lysis Buffer That Works
(1)
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Cell Signaling Technology
October 30, 2015
This cell lysis buffer is used to lyse cells under nondenaturing conditions. The lysis effect in relatively mild and we use it together with...
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xCT Antibody NB300-318 from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
October 29, 2015
This antibody was used to detect xCT expression in different breast cancer cell lines by western blot analysis. Two antibodies for xCT from ...
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Labeling of Human PBMCs with CD62L BV421 Antibody
(1)
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BioLegend
October 29, 2015
This CD62L antibody is included in a flow cytometry panel in order to identify L-Selectin expression on human PBMCs. Because multiple ...
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Cell Viability Stain
(1)
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BioLegend
October 29, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-...
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Great Way to Isolate Monocytes from PBMCs
(1)
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Miltenyi Biotec
October 29, 2015
With this product we achieved successful isolation of monocytes from human PBMCs with >90% purity of CD14+ monocytes and functionally active...
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Caspase-3 Apoptosis Kit
(1)
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BD Biosciences
October 29, 2015
I used 293T cells to induce apoptosis by oxidative stress. I check apoptosis by determining the active caspase 3 using this kit. The reason,...
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Flow Cytometric Method of Estimation of Glucose Uptake Using 2NBDG
(1)
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Cayman Chemical
October 29, 2015
2NBDG, a fluorescent analogue of glucose which represents amount of glucose uptake by cells. Measuring the amount of fluorescence by a ...
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Good ELISA Kit for Human IL-8 Protein Detection
(1)
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Thermo Fisher Scientific
October 29, 2015
This kit includes coating antibody, detection antibody, standards and streptavidin-HRP reagents. Need to order buffers and solution ...
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Ultra Low Temperature Freezer
(1)
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Eppendorf
October 29, 2015
This freezer is used to keep samples at -80 Celsius. The freezer has a large capacity and has not had issues with keeping temperature.
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GoBlot processor - Time saver!
(1)
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October 28, 2015
We have gotten four GoBlot processor machines so far Pros: 1. Time saving 2. Easy to handle 3. Convenient programs 4. Less buffer needed 5. ...
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Excellent Pre-Optimized Protein Estimation Kit
(1)
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October 28, 2015
Protein estimation is routine work that is generally performed prior to western blotting, SDS-PAGE etc. I use this kit for estimating the ...
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Labeling CD16 Positive Cells in Human PBMC Sample
(1)
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BioLegend
October 28, 2015
This is a good antibody for discriminating CD16 postive and negative cells such as monocytes, etc
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Bio-Rad Primers for RT-qPCR
(1)
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Bio-Rad
October 28, 2015
We are assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated by density ...
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Detection of Mouse Intestinal Macrophages by IFA
(1)
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BioLegend
October 28, 2015
To analyze the activation of mouse intestinal macrophages after experiment treatment, mice were gavaged with reagent and intestinal tissue ...
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Lab Rotator
(1)
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Glas-Col, LLC
October 27, 2015
Rotation module with interchangable heads (additional costs). This system is easy to use and works well even in prolonged time in the cold ...
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Brilliant Violet CD11b Antibodies
(1)
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BioLegend
October 27, 2015
This product was used to perform immunophenotyping on dog samples for diagnosis of lymphoma. Canine CD11b is expressed by granulocytes, ...
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Coverslip Mounting Solution With DAPI
(1)
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Thermo Fisher Scientific
October 27, 2015
This is a mounting solution for microscopy cell viewing. First of all, this mounting solution has DAPI in it, which brings up the price than...
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Good ELISA Kit for Human IL-6 Protein Detection
(1)
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Thermo Fisher Scientific
October 27, 2015
This kit includes standards, coating antibody, detection antibody and Streptavidin-HRP reagents. Need to coat the plate by yourself. Works ...
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Best FLAG Antibody for Immunoblot in Worms
(1)
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October 27, 2015
This is the most consumed antibody in our lab. The high specificity of this antibody always gives clean blots.
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Good Anti-FLAG Tag Antibody
(1)
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October 27, 2015
I used this antibody for studying protein-protein interaction between ESAT6 and novel peptides identified using dot-fat western technique.
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Detection of HIF1 Alpha
(1)
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BD Biosciences
October 27, 2015
I wanted to test the efficacy of HIF 1 alpha inhibitors in cells. I artificially induced upregulation of HIF 1 alpha using Cobalt chloride ...
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QC Beads
(1)
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October 26, 2015
We performed regular QC and checked alignment of all lasers on the FACS calibur instrument using these beads. They perform very well and ...
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CD8 FITC Antibody Review
(1)
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BioLegend
October 26, 2015
Mice are infected with the virus until it's latent, then splenocytes or PEC's are collected, RBC lysed, blocked, and stained.
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Best Antibody, Works Every Single Time
(1)
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Santa Cruz Biotechnology, Inc.
October 26, 2015
I use beta HRPO conjugated antibody as a loading control for all my western blotting. I look for several cell cycle molecules in diffferent ...
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Mouse Anti-CD8 Antibody
(1)
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BioLegend
October 26, 2015
CD8 is expressed on MHC-I restricted T cells. Since this is a lineage marker, I wanted to see if it is easily detected by a weak fluorophore...
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Good Anti-CD44 Antibody for FACS
(1)
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BioLegend
October 26, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Anti Mouse CD3 PB for Flow Cytometry
(1)
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BioLegend
October 26, 2015
I used this to identify the CD3+ T Cell population in the mouse spleen to use for downstream research.
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Good Antibody for CD4 on Dim Fluorophore
(1)
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BioLegend
October 26, 2015
CD4 is expressed on MHC-II restricted T cells within lymphocytes. Since the population is well-characterized, I wanted to test if it can be ...
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CoolCell Cell Freezer
(1)
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Zen-Bio, Inc.
October 26, 2015
This container is used to freeze down cell lines at -1 degree Celsius per minute, which helps with increasing cell viability. These do not ...
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Phospho-Akt (Ser473) Antibody from Cell Signaling
(1)
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Cell Signaling Technology
October 23, 2015
This antibody was used to detected changes of Akt phosphorylation in response to cetuximab treatment in a time dependent manner.
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PE Anti-Mouse IFN-gamma
(1)
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BioLegend
October 23, 2015
Our purpse was to determine allergic inflammatory response in mouse lung. This product was used to identify IFN gamma producing lymphocytes ...
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Co-Expression of Positive Activation Marker CD38 and Negative or Exhaustion Marker PD-1 in CD8+ T Cells
(1)
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BioLegend
October 23, 2015
CD38 is considered a marker of immune activation expressed in T, B and NK cells. The role of CD38 in CD8+ T cell function against viral ...
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Ribosomal Protein S6/RPS6 Antibody for Western blotting
(1)
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Novus Biologicals, a Bio-Techne Brand
October 23, 2015
To study the effects of mToR inhibitor on Ribosomal Protein S6/RPS6 phosphorylation
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Good CD19 Antibody
(1)
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BioLegend
October 23, 2015
This product was used while trying to distinguish B cell populations in the peritoneum of latently infected mice. We selected this product ...
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CD8a+ Staining of Murine Lymphocytes
(1)
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BD Biosciences
October 23, 2015
For T cell inducing vaccines, their immunogenicity is investigated following immunisation. PBMCs isolated from mice post immunisation are ...
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ASCL-4 for Fractionation Purity
(1)
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Santa Cruz Biotechnology, Inc.
October 23, 2015
ASCL-4 was used to test the purity of a subcellular fractionation experiment. This particular antibody was used to monitor the purity of the...
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CD14 FITC Antibody Using Human PBMCs
(1)
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BioLegend
October 22, 2015
Search for a reliable antibody for identifying CD14 positive cells from PBMCs such as monocytes etc. FITC-conjugated antibodies proved to ...
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Good ELISA Kit for Detecting Human MMP-13 Protein
(1)
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RayBiotech
October 22, 2015
This kit works well in detecting the MMP-13 protein concentration in the culture media of human annulus fibrosus cells. The standard curve...
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Good Proliferation Assay
(1)
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October 22, 2015
I study immune response during asthma and use cell proliferation assays quite often. I use anti-BrdU staining kit to study proliferation of ...
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Nice Antibody for Western Blot
(1)
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Cell Signaling Technology
October 22, 2015
This antibody is one of the best antibodies that I used for apoptotic molecules so far. No background, no non specific bands ...nothing......
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Good Anti-CD25 Antibody for FACS
(1)
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October 22, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-...
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Fixable Live Dead Stains
(1)
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Thermo Fisher Scientific
October 22, 2015
The LIVE/DEAD Fixable Dead Cell Stain Kit can be used to determine the viability of cells prior to the fixation and permeabilization ...
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Mouse IL-10 ELISA Kit to Determine Picogram Levels
(1)
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BioLegend
October 22, 2015
Mice sera injected with DNA were assessed for the production of IL-10 at the end of 10 days of experiment. 100ul of 1:2 diluted serum ...
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Good Secondary Antibody
(1)
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Jackson ImmunoResearch Laboratories, Inc.
October 22, 2015
I am using this secondary antibody for years, in WB and ELISA. Gives good signals with low background
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Detecting Mouse Serum IgG Antibody
(1)
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BioLegend
October 21, 2015
This product was used to test if our pseudovirus can induce anti-target protein L1 antibody response after vaccination. Mice were immunized ...
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