Cell Signaling Technology
p53 Antibody
9282
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This antibody was used to detect total expression of p53 in response to ionized radiation treatment in HN5 human HNSCC cells by western blot.
Western Blot
Human HNSCC cell line whole cell lysate
1:2000, overnight at 4 degrees
5% milk
1:2000, 1 hour at RT
NA
Enhanced Chemiluminescence Detection Kit
In HN5 cells, this antibody detected total p53, which I used as a control for phospho-p53 at S15 site.
None
Detects specific band and the signal is strong
This is a perfect antibody for western blot.