Li-Cor
IRDye® 680RD Donkey anti-Mouse
925-68072
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This antibody is used to probe for mouse primary antibodies. The secondary is made to be used in the Odyssey imaging system and can be use alone or in duplex western blots with IR800 secondaries.
Western Blot
Epithelial cells
1:5000, overnight, 4 degrees
Odyssey® Blocking Buffer
1:5000, 1 hr, room temperature
NA
Odyssey Imaging System
The antibody was used to detect primary mouse beta-actin antibody. The secondary shows a single band at the correct molecular weight. This is a good antibody to use with an Odyssey imaging system.
None
Low background signal
Can only be used with Li-Cor system
This is a good secondary antibody for Odyssey imaging systems.