Staining of Neutrophils in Mouse Spleen

December 08, 2015

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-mouse Ly-6G

Catalog Number:

127633

This antibody was used to identify neutrophils among mouse splenocytes to analyze cell numbers and exclude these cells from analysis of myeloid cell types.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400, 15min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

This antibody has a good signal to noise ratio making it easy to identify neutrophils among mouse splenocytes. BV510 is bright and has little overspill into other "classical" flow channels.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Using the violet laser for less compensation issues

The Bad

None

The Bottom Line

Good antibody to distinguish neutrophils from other splenocytes.

Share your experience with other scientists. Write a Review! »

Join the discussion