Miltenyi biotec
CD4 Microbeads L3T4 mouse
130-049-201
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We aim to co-inject bone marrow and CD4 splenocytes into P0 mice to evaluate GVHD response as compared to bone marrow alone. We selected these beads as we have had previous success with isolation kits from Miltenyi.
Affinity Purification
Splenocytes from 2 week old mice
15 min at 4 degrees C as instructedy by manufacturer
No block used
NA; however we did confirm isolation using anti-CD4 APC from eBioscience; 30 min 4 deg C
NA
Flow Cytometry confirmed isolation; FACSCalibur
Final sample was ~93% CD4+ and ~2% CD8+ by FC. We did notice that the sample had fewer cells than expected from previous literature ( ~5% as compared to 20% of splenocyte MNC were CD4+).
None
Familiarity, ease of use
Washing and Titrating steps time consuming
Would buy again as you save a step as compared to CD4-fluorphore conjugated beads, but if I wanted to ensure purity I may run through a second time.