Thermo Fisher Scientific
Turbo DNA free Kit
AM1907
Bacterial RNA samples extracted from Trizol method were treated with Turbo DNase enzyme following Turbo DNA free kit protocol. Absence of genomic DNA was confirmed by diagnostic PCR of the Turbo DNase treated samples. This enzyme removed genomic DNA efficiently from precious RNA samples in solution.
Real time PCR using bacterial RNA samples
Total RNA from E. coli
Bacterial RNA samples extracted from Trizol method were treated with Turbo DNase enzyme in solution following kit's easy to perform instructions. 2 microliters of enzyme was used for a total of 50 micrograms of RNA in a 50 microliter reaction volume.
None
Efficient removal of genomic DNA from total RNA samples which was evident from diagnostic PCR.
Kit is very easy to perform and takes less time.
Highly recommended for treating the RNA samples to remove genomic DNA if it is intended for qPCR or NGS applications.