MTA2 Antibody from Bethyl Laboratories

Biological Sciences
Columbia University
Postdoc

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Company:

Bethyl Laboratories

Product Name:

MTA2 Antibody

Catalog Number:

A300-395A-T

I am investigating functions of chromatin modification complexes in RNA expression. The goal of this experiment was to test the specificity of the antibody to detect MTA2 expression in 293 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293 Whole Cell Extract

Primary Incubation

1:1000 dilution overnight in 5% milk buffer at 4C

Blocking Agent

5% milk in TBS

Secondary Incubation

1:10000 anti-rabbit secondary at RT for 1 hour

Tertiary Incubation

NA

Detection

ECL

Results Summary

The results show two bands between 50-75kDa.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bands appeared at the correct molecular weight

The Bad

Lower one may be a non-specific band

The Bottom Line

This antibody works for MTA2 detection

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