PE/Cy7 Anti-Mouse/Human CD11b Antibody

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse/human CD11b Antibody

Catalog Number:

101215

In order to study immune cell influx during a self limiting inflammatory response in the lung, LPS was instilled into mouse lungs. 24 hours later, brohchoalveolar lavage was performed to isolate airway cells. CD11b antibody was used as a second marker for neutrophils, as well as a phenotypic marker for infiltrating monocytes and alveolar macrophages. This antibody was recommended by a colleague.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse primary immune cells

Primary Incubation

1:200 in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

Clearly identified CD11b positive cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear separation of positive and negative cells

The Bad

None

The Bottom Line

Super clean antibody. Highly recommended.

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