PE/Cy7 anti-mouse CD45

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD45

Catalog Number:

103113

Cells from brohchoalveolar lavage of LPS treated mice (24h post instillation, 5 micrograms) were stained for flow cytometry. CD45 antibody was used to select hematopoietic cells to further characterize the recruited cell population. This product was recommended by a colleague.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse primary cells from lung airspace (BAL)

Primary Incubation

1:100 in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

Clearly identified CD45 cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very clean antibody

The Bad

None

The Bottom Line

Highly recommended for staining mouse primary immune cells

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