T202/Y204-Phosphorylated Erk Antibody from Novus Biologicals

March 04, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

T202/Y204-phosphorylated Erk Antibody

Catalog Number:

NBP1-19924

This antibody is used to detect phosphorylation of Erk at T202/Y204 site in response to ERK inhibitor U0126 in MDA-MB-231 human breast cancer cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody clearly detect changes of phosphorylated ERK in response to ERK inhibitor U0126 by western blot.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects specific band with clean background

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

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