Sox2 Antibody from Novus Biologicals

March 02, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Sox2 Antibody

Catalog Number:

NB110-37235

This antibody was used to detect Sox2 expression in breast cancer cell lines in response to paclitaxel treatment by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody detects Sox2 expression in breast cancer cell lines with some non-specific bands.

Additional Notes

None

Related Categories

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Summary

The Good

Can detect specific bands

The Bad

Has a lot of non-specific bands, Signal becomes weak after a couple of reuses

The Bottom Line

This antibody can be used to detect the expression of Sox2 in breast cancer cells. However, try to use the antibody as fresh as possible since the signal becomes weak after a couple of reuse.

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