Cell Signaling Technology
Phospho-Akt (Thr 308) Antibody
9275S
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This antibody was used to detect Akt phosphorylation at Thr 308 site in A431 cells
Western Blot
Human Cancer Cells whole lysates
1:1000, overnight, 4 degrees
Non-fat milk
1:2000, 1 hour, RT
NA
Enhanced chemiluminescence detection kit
This antibody detects changes of phosphorylation of Akt at T308 in response to 1, 9-Pyrazoloanthrones treatment in A431 cells.
None
Can detect specific bands
Background is dirty
This antibody works to detect phosphorylated Akt at Thr 308 site, although the background is kind of dirty.