PerCP Anti-Mouse CD45 Antibody

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP anti-mouse CD45 Antibody

Catalog Number:

103129

In order to study immune cell influx in infection-related lung inflammation, LPS was instilled in mouse lungs by intranasal instillation. Bronchoalveolar lavage was performed 24 h later, and the cells were isolated and stained for flow cytometry. CD45 antibody was used to select hematopoietic cells to further characterize the recruited cell population. We used this product because of their robust staining and clear results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary mouse BAL cells

Primary Incubation

1:100 in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

Clearly identified CD45 cells. It was very good for compensation control too.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clean antibody

The Bad

None

The Bottom Line

Very good for staining primary mouse immune cells

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