FACSAria II Flow Cytometer

University of Montana
DBS
Graduate Student

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Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FACSAria II Flow Cytometer

Catalog Number:

650033

We are assessing consequences of Aryl hydrocarbon Receptor (AhR) activation in dendritic cells. Specifically for these experiments we were assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated with Lympholyte M from whole bone marrow suspensions and grown in the presence of GM-CSF for 5-7 days. We then use flow cytometry and RT-PCR to assess changes in dendritic cells after AhR activation.

Experimental Design and Results Summary

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Summary

The Good

Software is fairly easy to navigate

The Bad

Clogs are a common issue with the smaller nozzles.

The Bottom Line

This machine is great for cell sorting, but slow if flow cytometry is its main use.

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