Cell Signaling Technology
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
4060
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Browse Similar Products: Phospho-AKT Western Blot Antibodies
Our lab works with all types of cancers, and we're often blotting for phospho-AKT to see downstream effects of mTOR manipulation. We gave this Cell Signaling antibody a chance on a recommendation from another lab, and it's been great.
Western Blot
786-0 Cell Lysates
1:1000 Dilution Overnight in 5% BSA in 1X TBS-T
5% Milk in 1X TBS-T
1:5000 dilution of HRP-conjugated Rabbit secondary in 5% Milk in TBS-T for 1 hour at RT
None
ECL/FEMTO --> Autoradiography Film via HRP-Conjugate Rabbit Secondary Antibody
I've been very pleased with this antibody. It's not always easy to get a clean blot for this phospho site on AKT, but this antibody has been able to deliver clean, crisp results even at very low protein levels. I recommend it to anyone that doesn't want to have to waste time optimizing their protocols.
Clean, Works Great
Expensive
This antibody is worth it - just make the purchase and you'll be pleased with the high-quality products offered by Cell Signaling Technology.