BD Biosciences
APC Rat anti-mouse FR4 (folate receptor 4)
560318
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We wanted to sort live CD4 T regulatory cells for functional assays and one of the markers we used to identify Tregs is folate receptor 4 (FR4).
Flow Cytometry
Splenocytes
+4C, 30 mins
Anti-CD16/32
NA
LSR II
This antibody gives a decent separation between positive population and background. It is highly expressed in the CD25+Foxp3+ population but not CD25-Foxp3- cells. When used in combination with other Treg-associated markers such as GITR, CD25 and CD127 this antibody can be useful to isolate live Tregs.
None
Good separation
not specific for Tregs. Also expressed by antigen-experienced T cells.
Useful marker for Treg when used in combination with other Treg-associated markers