Good Antibody for IgM Detection

March 03, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE mouse anti-human IgM

Catalog Number:

555783

Surface IgM was assessed on human B cells to look for class switching.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD19 B cells

Primary Incubation

30 min on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

High IgM expression was detected on B cells.

Additional Notes

None

Related Categories

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Summary

The Good

Bright enough

The Bad

None

The Bottom Line

Good antibody for bright detection of IgM

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