Great, Reliable ELISA

University of California, San Diego
Department of Pediatrics
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse IL-1 alpha DuoSet ELISA

Catalog Number:

DY400

This is a consistent and easy to use ELISA. We detected changes in tissue cytokines without a problem.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Murine lung homogenate supernatant

Protocol Overview

Mice were infected with M. tb and lungs were collected in PBS-tween 80. Lungs were homogenized and supernatant was diluted 1:3 in assay diluent, then analyzed as per protocol guidelines

Tips

Collect sample in low volume and dilute as needed to get within standard curve.

Results Summary

Reliable and easy to use. Detected changes in tissue cytokines without a problem.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use, consistent results

The Bad

100uL sample was used for assays. This is fine for something like tissue homogenate, but may not work well if samples are limited, such as serum analysis, especially if you are analyzing multiple cytokines.

The Bottom Line

Great product, would use again.

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