Bright Staining with HLA-DR Antibody

March 07, 2016

BlueskyReddit
Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BV650 anti-human HLA-DR

Catalog Number:

564231

This HLA-DR antibody was used to assess surface MHC expression on B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD19 B cells

Primary Incubation

30 min on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

The staining on B cells is consistent with this antibody. You can detect high MHC expressing B cells compared with the isotype.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright enough

The Bad

None

The Bottom Line

Good reliable antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion