Bright Staining with IgD Antibody

March 04, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Per CP Cy5.5 anti-human IgD

Catalog Number:

561315

Surface IgD was assessed on the surface of CD19+ B cells to look for class switching.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD19 B cells

Primary Incubation

30 min on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

The antibody gives high staining of IgD on the cell surface.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright enough

The Bad

None

The Bottom Line

Good antibody for straightforward detection of IgD on the cell surface.

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