Excellent Phospho p53 Antibody

March 08, 2016

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p53 (Ser15) Antibody

Catalog Number:

9284

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates

Primary Incubation

1:1000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X PBS

Secondary Incubation

1:3000 at room temperature for 2 hour

Tertiary Incubation

NA

Detection

Western Blot

Results Summary

High level of p53 activation was observed with stimulation with antigen.

Additional Notes

None

Related Categories

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Summary

The Good

Antibody is highly specific and gives minimum background

The Bad

None

The Bottom Line

Reliable Phospho p53 Antibody with minimum background problem

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