Very Good Antibody for Mouse Ly6c

March 04, 2016

Internal Medicine
University of California Medical Center
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti- mouse FITC Ly6c

Catalog Number:

128005

I use this antibody in flow cytometry to study the distribution of ly6c marker in mouse organs under various conditions of inflammation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse peripheral lymph node cells

Primary Incubation

1:300 in Fc block in dark at 4C

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FITC channel based

Results Summary

Very good detection is possible with this color combination on Ly6c even under low expression levels

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good color, low leakage

The Bad

None

The Bottom Line

Very good for flow cytometry

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