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Date Published
IHC Staining of Alexa Fluor® 488 On Optic Nerve Section
(1)
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BioLegend
May 25, 2022
Fixed (4% PFA) and cryoprotected (sucrose 10-30%) optic nerves were permeabilized with PBS-Triton 0.25 % for 20 min and blocked with PBS - 3...
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Excellent Antibody for Western Blot
(1)
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Cell Signaling Technology
May 25, 2022
I was interested in evaluating RAS pathway, especially ERK on A549. I stimulated the cells IGF-1 for 24 hours and lysed my cells with 100ul ...
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Antibody Stains hCD68 Very Well
(1)
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BioLegend
May 24, 2022
We wanted to phenotype human macrophages in an in vitro culture, so we used hCD68 as a differentiation marker to compare to monocytes. This ...
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Excellent GAPDH Antibody
(1)
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Cell Signaling Technology
May 24, 2022
I have been using this GAPDH antibody for 7 years and very satisfied with it's performance as a loading control in Western Blot. I tested in...
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Nice Hexokinase II Rabbit mAb
(1)
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Cell Signaling Technology
May 24, 2022
I used this antibody in studying glycolysis pathway in bone-marrow derived macrophages cells. This antibody is consistent. I have used in ...
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Good 4E-BP1 Antibody for Western Blot
(1)
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Cell Signaling Technology
May 24, 2022
I stimulated epithelial cells for 24 hours with TGF-b and I transfected eIF4 siRNA for 24 hours with starvation medium (0.5% FBS). I diluted...
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Good Sonifier
(1)
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Branson Ultrasonics Corp.
May 24, 2022
Our lab studies brain endothelial cells and pericytes. We use this sonicator to homogenize the mouse brain for brain microvascular ...
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Reliable Glucose Assay Kit
(1)
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Abcam
May 24, 2022
I used this to evaluate the glucose consumption on my cells stimulated with TGF-b. I followed the passage as written in the kit and I build ...
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Good SirT3 Antibody from Cell Signaling
(1)
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Cell Signaling Technology
May 24, 2022
I seed epithelial cells (lung cancer cells) for 24 hours and I treated with eIF4E siRNA for 24 hours. I evaluated SirT3 expression with ...
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Good Antibody for cGAS Localization
(1)
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Cell Signaling Technology
May 24, 2022
We wanted to determine whether cGAS was localized to micronuclei in head and neck cancer, as this was previously described in other cancer ...
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Good Phospho-Stat3 Antibody for Western Blot
(1)
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Cell Signaling Technology
May 23, 2022
I seeded epithelial cells with 10% FBS. The day after, I replaced the medium (starvation medium, 0.5%) and treated it with eIF4E siRNA for ...
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Great CD45 Antibody from BioLegend
(1)
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BioLegend
May 23, 2022
The overall objective of my PhD studies will be to elucidate how diets, particularly KD, affect the immune response in adaptive and ...
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Great Lineage Marker to Identify iLCs
(1)
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Thermo Fisher Scientific
May 23, 2022
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Great HIF-1a Antibody for Western Blot
(1)
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Cell Signaling Technology
May 23, 2022
I exposed my endothelial cells for 24 hours in hypoxia chamber (O = 1% and CO2 = 5%). I diluted the primary antibody up to 1:1000 and I got ...
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Great Fluorescent Beads to Study Macrophage Phagocytosis
(1)
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Thermo Fisher Scientific
May 23, 2022
The overall objective of my PhD studies will be to elucidate how diets, particularly KD, affect the immune response in adaptive and ...
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Time Saving Genotyping Service
(1)
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Transnetyx Inc
May 23, 2022
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Great Loading Control Antibody for Western Blot
(1)
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Cell Signaling Technology
May 23, 2022
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Good Phospho-mTOR Antibody with Optimization
(1)
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Cell Signaling Technology
May 23, 2022
I seeded epithelial cells with 10% FBS. The day after I replaced the medium (starvation medium, 0.5%) and I treated with eIF4E siRNA for 24 ...
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Fast Sanger Sequencing Service
(1)
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May 23, 2022
The overall objective of my PhD studies will be to elucidate how diets, particularly KD, affect the immune response in adaptive and ...
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Good Cytochrome Oxidase Activity Assay Kit
(1)
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May 20, 2022
Our research is to evaluate mitochondrial function in mouse liver tissues. We used this kit to get the cytochrome oxidase activity. This kit...
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Good Secondary Antibody for Western Blot
(1)
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Cell Signaling Technology
May 20, 2022
I used this antibody several times and I always got great results. I diluted 1:10000 with 5% BSA in TBST and I incubated my membranes from 1...
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Great Anti-CD3 Antibody for Immunofluorescent Staining for Paraffin Tissue
(1)
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Abcam
May 20, 2022
We used this antibody to study the T cell infiltration in a TUBO breast cancer mouse model. This anti-CD3 antibody has worked well for ...
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Easy to Handle Assay Kit with Reliable Results
(1)
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Thermo Fisher Scientific
May 19, 2022
We use the Pierce™ BCA Protein Assay Kit frequently to determine the protein concentration of our whole cell lysates (different human cell ...
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Very Bright CD4 Antibody
(1)
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May 19, 2022
Our lab continues to study human lymphocyte populations, including CD4+ and CD8+ T cells. In a previous review, I showed how CD4 FITC worked...
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D-Luciferin for In Vitro and In Vivo Bioluminescent Assays
(1)
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May 19, 2022
Our goal is to generate MDA-MB-231 murine bone metastatic model. We intracardiac injected MDA-MB-231 cancer cells transfected with a plasmid...
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Great GelRed® Nucleic Acid Gel Stain
(1)
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Biotium, Inc.
May 19, 2022
The background of this research is to evaluate nanoparticle and siRNA binding ability. We used GelRed nucleic acid stain as precast in ...
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Great Low Glucose Amino Acid-Free Medium Powder
(1)
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United States Biological
May 19, 2022
We are trying how the compositions of amino acids affect a peptide hormone's function in primary adipocytes. This is great DMEM powder ...
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Great Collagenase for Hepatocytes Isolation
(1)
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MilliporeSigma
May 19, 2022
We used this product for hepatocytes isolation from mice. We needed to pre-test due to different enzyme activity.
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Great Mycoplasma Removal Agent
(1)
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MP Biomedicals
May 19, 2022
At certain points, we've had to deal with some mycoplasma contamination in our cell cultures. This removal agent works great to keep our ...
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Bright CD5 Antibody for T Cells and CLL
(1)
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May 19, 2022
CD5 is typically used for identification of T cells; however, in our lab we also use it to identify chronic lymphocytic leukemia/small cell ...
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Good COX IV Antibody for Western Blot
(1)
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Cell Signaling Technology
May 18, 2022
I used this antibody to evaluate the mitochondria in my cells. My research is focused on the SMAD pathway activated by TGF-b. This antibody ...
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Great Integrin Beta 2 Antibody for Western Blot
(1)
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R&D Systems, a Bio-Techne Brand
May 18, 2022
I used this antibody to evaluate the expression of CD18 in inflammation sites. This antibody worked very well, resulting in a clear band and...
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Great Anti-Desmin Antibody
(1)
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Abcam
May 18, 2022
This is a nice antibody. We used it as a smooth muscle cell marker and checked expression on Rat pulmonary smooth muscle cells. The signal ...
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High Quality Green Supermix
(1)
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Bio-Rad
May 18, 2022
This green supermix from Bio-Rad is always easy to handle and works well for our purposes. While it is a little more expensive compared to ...
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GenDEPOT Xpert 2 Prestained Protein Marker Western Blot marker
(1)
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May 18, 2022
Our lab uses this protein marker for almost all our Western Blot studies. It's ready to use and very convenient. It has a broad range (11-...
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Good TRIzol™ Reagent
(1)
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May 18, 2022
We use this TRIzol reagent from Thermo Fisher for mRNA isolation. For our work, we isolated mRNA from adipose tissue with 1mL of the ...
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Great Dispase for Adipose Fibroblast Isolation
(1)
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May 18, 2022
We are studying a peptide hormone's potential function on adipocytes. This dispase II is great in isolating adipose-derived stromal vascular...
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Great Vector® TrueVIEW® Autofluorescence Quenching Kit
(1)
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May 17, 2022
We use TrueView to reduce autofluorescence in brain, liver and heart tissues. TrueView (mounting media with DAPI) was added after all ...
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Great CD31 Antibody for Flow Cytometry
(1)
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Fisher Scientific
May 17, 2022
Endothelial cells were isolated from mouse brain tissue for in vitro culturing. CD31 Rat anti-mouse PE antibody was used to assess ...
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Kit Didn't Work, but Likely User Error
(1)
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R&D Systems, a Bio-Techne Brand
May 17, 2022
Tried to use for media from astrocytes activated by amyloid beta. I did not detect any TNF-a, however, I discovered after the fact that ...
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Great Histone H3 Antibody for Western Blot
(1)
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Cell Signaling Technology
May 17, 2022
We used this antibody to detect Histone H3 expression in the HeLa cell line by western blot analysis. The antibody works well at the ...
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Great Kit to Analyze Protein Interactions
(1)
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MilliporeSigma
May 17, 2022
I used this with HEK293 cells to detect protein protein interactions. Kit is easy to use and the company provides a lot of troubleshooting ...
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Good Desalting Columns
(1)
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May 17, 2022
We are purifying recombinant proteins from cells using His-columns. These desalting columns from Cytiva work great to remove the remaining ...
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Good Protein Gels for Western Blot
(1)
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Bio-Rad
May 17, 2022
I used these gels several times for my western blot. The quality is always good. I have never had any gels break or any other problems. ...
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Could Not Detect IL-6 in Samples
(1)
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MyBioSource.com
May 17, 2022
Used this ELISA kit with media from activated (via amyloid beta) human astrocytes. Could not detect IL-6 even after concentrating.
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Excellent Siliconizing Reagent
(1)
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MilliporeSigma
May 16, 2022
Clot retraction assay was performed between WT and PACSIN knockout mice to look at the quality of clot formed and the plasma remaining after...
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Great Fibronectin Antibody
(1)
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MilliporeSigma
May 16, 2022
Anti cellular fibronectin antibody was used to compare fibronectin levels in WT and PACSIN2 knockout mouse plasma samples in Western blots ...
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ELISA Kit Worked Great for Astrocytes
(1)
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R&D Systems, a Bio-Techne Brand
May 16, 2022
Used this ELISA kit for media from human astrocytes after amyloid beta exposure. This kit was the only one sensitive enough for our needs, ...
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Good FBS for Cell Culture
(1)
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R&D Systems, a Bio-Techne Brand
May 16, 2022
I used this FBS for primary hepatocytes from mouse, human fibroblast, and RT4 cell lines. Working well in primary cells, adipocytes, ...
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ELISA Kit Works Well for Mouse Samples
(1)
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Abcam
May 16, 2022
Used this ELISA kit for mouse serum samples and it worked well. The kit is for human transferrin but it works just fine with mouse.
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Western Blot
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Cell Fractionation
Immunoassay
In Vitro Transcription
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3D Imaging
RNAi
ELISPOT
PCR Cloning
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Protein Modification
DNA Analysis
Expression
Cell-Based Assay
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In Vivo Testing
Protein Labeling
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Human
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Adipose
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Cell Type
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Adipocyte
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Microvascular Endothelial Cell
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Platelets
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Tumor Cells
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HeLa
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A431
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N2a
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