Phospho-Rb (Ser807/811) Antibody for Western Blot

June 24, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-Rb (Ser807/811) Antibody

Catalog Number:

9308

Clone Number:

N/A

This antibody was used to detect phospho-Rb (retinoblastoma protein) expression from human cell lysates in western blot analysis. Rb regulates cell proliferation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:1000, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-rabbit IgG HRP (#HAF008, R&D systems), 1:5000

Tertiary Incubation

None

Detection

Chemiluminescent

Results Summary

This antibody provides consistent bands at 110 kDa in proliferative cells. The dilution in BSA/TBS can be reused twice. 25 ug/mL protein was used.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Sensitive, consistent.

The Bad

Sometimes the signal is weak.

The Bottom Line

Overall it is a good antibody that provides reproducible results.

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