Accessible Anti-Cyclin A Antibody (B-8) for Western Blot

June 27, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnologies

Product Name:

Anti-cyclin A antibody (B-8)

Catalog Number:

sc-271682

Clone Number:

N/A

Cyclin A is a crucial component of the cell-cycle machinery. This antibody was used to detect cyclin A protein levels in human cell lysates through Western Blot analysis

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:1000, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-mouse IgG HRP (#HAF007, R&D systems); 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

Bands consistently appear at 45 kDa. Sometimes the signal is weak and it can only be detected with higher exposures. Protein loading: 25 ug/mL. Dilution in BSA/TBS cannot be reused twice.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accessible antibody.

The Bad

Sometimes the signal is weak or the Ab does not work; cannot be reused.

The Bottom Line

Accessible but not always work.

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