Anti-GFAP for Staining Astrocytes in Murine Brain Tissue Sections

June 24, 2022

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences (BD Pharmingen)

Product Name:

Purified Mouse Anti-GFAP Cocktail

Catalog Number:

556330

Clone Number:

Multiple (cocktail)

Info:Supplier Page View Product Specs

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We use BD's murine anti-GFAP cocktail to stain astrocytes in brain sections obtained from our mouse models of neurodegeneration. The cocktail is relatively easy to optimize and provides clear, consistent staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine brain tissue sections

Primary Incubation

1:500; overnight at 4 degrees C.

Blocking Agent

BSA

Secondary Incubation

Goat anti-mouse @ 1:500; 1 hour at RT.

Tertiary Incubation

N/A

Detection

Fluorescence or confocal microscopy.

Results Summary

Using this antibody, astrocyte cell bodies and branching/ramifications are highly resolved by both fluorescent and confocal imaging platforms.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High resolution results; Overall, antibody cocktail works quite well.

The Bad

Optimization required and highly dependent on starting material.

The Bottom Line

We recommend this product for staining astrocytes if visualization of both bodies and branching is required -- the resolution is more than sufficient.

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