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Date Published
Excellent Beta Actin Antibody
(1)
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Cell Signaling Technology
May 04, 2022
I used this antibody with the aim to evaluate mitochondria biogenesis pathway in A549 and MRC5 cells. After applying the conditions ...
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RT/4C Centrifuge for 15 & 50mL Tubes and Multi-Well Plates
(1)
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Thermo Fisher Scientific
May 04, 2022
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Affordable Blood Ketone Meter
(1)
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Abbott Laboratories
May 04, 2022
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Specific COV IV Antibody but Blurry Bands
(1)
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Cell Signaling Technology
May 04, 2022
Antibody is strong and specific in all my cells tested in WB (MRC5, SHSY5Y, A549, primary mouse CD8+ T cells). I used at a dilution 1:1000 ...
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Good Antibody For WB and PLA
(1)
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Cell Signaling Technology
May 04, 2022
Used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution. Works for western blot using human iPSC-derived Ecs ...
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Pretty Good DMT1 Antibody for WB
(1)
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Alpha Diagnostic International Inc
May 04, 2022
This antibody works for western blot using human iPSC-derived ECs at 1:1000. Produces more non-specific bands than others, but does give ...
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Really Good FKBP12 Antibody for Western Blot
(1)
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Abcam
May 04, 2022
I used this Ab for detecting FKBP12 in the skeletal muscle of FKBP12KO and WT control mice. This Ab is really good for western blot.
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Recommended FN1 Antibody
(1)
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Cell Signaling Technology
May 04, 2022
I used this antibody after inducing Epithelial Mesenchymal Transition in A549 with TGFb. I was able to accurately evaluate fibronectin ...
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Very Strong Band with OPA1 Antibody
(1)
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Cell Signaling Technology
May 03, 2022
Used at a concentration of 1:1000, it provides very strong bands. I used this antibody in SHSY5Y with the purpose to evaluate mitochodria ...
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Wrong Band from HRP Antibody
(1)
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BioLegend
May 03, 2022
Use this for IP-western blot for my mouse sample, but shows clear band with wrong molecular weight when comparing to the other two validated...
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Great Assays to Measure BHB and AcAc
(1)
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MilliporeSigma
May 03, 2022
I'm trying to understand how different types of diets influence and alter the host's immunological response to disease.
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Okay DMT1 Antibody for WB, Slightly Faint
(1)
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Abcam
May 03, 2022
Used for Western blot using human iPSC-derived Ecs at 1:1000. Bands were very difficult to detect after treatment.
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Good Plasmid Isolation Kit
(1)
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May 03, 2022
We routinely use this kit to isolate plasmids for cell culture work. We study the blood brain barrier and basement membrane in the CNS.
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Efficient Parkin Antibody
(1)
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Abcam
May 03, 2022
Very clean band after diluting 1:1000 in 5% BSA/TBST 1x. I used this antibody with the aim to evaluate mitophagy in SHSY5Y cells after ...
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Great Ready-to-Use Antibody, No Background Bands
(1)
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Cell Signaling Technology
May 02, 2022
Used for western blot from cell lysate with RIPA buffer and Licor secondary. No background bands. Very clear detecting endogenous protein.
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Great DMT1 Antibody for WB
(1)
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MilliporeSigma
May 02, 2022
This DMT1 antibody was used for WB using human iPSC-derived ECs at 1:1000. While there were a few non-specific bands, we recommend this ...
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Good CD18 Antibody for IF
(1)
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Thermo Fisher Scientific
May 02, 2022
We are interested to study tissue-resident macrophages in various tissues. As a positive control for CD18, we stained the mouse spleen.
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Reliable Assay Kit
(1)
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May 02, 2022
I have ran this assay many times over the course of the last six months and have not had issues with the test reliability or during any step...
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Great and Easy To Use for RT-qPCR
(1)
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Thermo Fisher Scientific
May 02, 2022
I apply large-scale multi-omics assays in human samples for basic and translational biomedical research, from scRNA-Seq to epigenomics and ...
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Great Clec4f Antibody for IF
(1)
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BioLegend
May 02, 2022
We are interested to study tissue-resident macrophages in various. For this, we wanted to identify the liver-resident Kupffer cells.
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Good SIRPa (CD172a) Antibody for IF
(1)
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BioLegend
May 02, 2022
We are interested to study tissue-resident macrophages in various organs. As a positive control for SIRPa we stained the spleen.
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Great CD31 Antibody for IF
(1)
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R&D Systems, a Bio-Techne Brand
May 02, 2022
We are interested to study tissue-resident macrophages and their interplay with endothelial cells.
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Best Antibody for PLA
(1)
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Proteintech Group Inc
May 02, 2022
This antibody was used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution.
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Time Course Effect of Chemoattractant on GPR75 Expression
(1)
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Thermo Fisher Scientific
April 29, 2022
GPR75 is a G protein coupled receptor 75 that is encoded by the GPR75 gene. GPRs are present on the cell surface receptors that activate ...
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Good CD38 Antibody for WB
(1)
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Thermo Fisher Scientific
April 29, 2022
CD38 is present in many immune cells such as white blood cells, CD4+, CD8+, B lymphocytes and natural killer cells. It also functions in ...
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Excellent Product for Protein Quantification
(1)
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April 29, 2022
The aim of the experiment was to quantify plasma proteins from different WT and PACSIN KO mice to be later used for cytokine array. The ...
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ß3 Tubulin for IF
(1)
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BioLegend
April 29, 2022
We are interested in studying tissue-resident macrophages in the kidney and their interactions with other stromal cells. For this, we wanted...
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Great RT-PCR Kit From Single Cells
(1)
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Thermo Fisher Scientific
April 29, 2022
This is great kit of RT-PCR from single cells (works well for cells isolated from cell culture or animals). We were interested in ...
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Good Insulin Antibody for Immunofluoresccence Microscopy
(1)
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Thermo Fisher Scientific
April 29, 2022
We are interested to study tissue-resident macrophages in various, including the pancreas. For this, we wanted to identify the endocrine and...
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Good JAK1 Antibody for Total Protein Quantification
(1)
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Cell Signaling Technology
April 29, 2022
I generated a JAK1 knockout cell line and wanted to confirm that the JAK1 protein was completely depleted from these cells using a Western ...
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Pretty Straightforward Protein Synthesis Kit
(1)
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April 28, 2022
Good alternative to radioactive ways of measuring protein synthesis. Straightforward OPP puro treatment and then subsequent fixation and ...
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Great Genomic DNA Amplification Kit
(1)
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Agilent Technologies
April 28, 2022
We were interested in understanding the transcriptome response of immune cells in mice to a peptide hormone. This kit is very realizable in ...
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Great cDNA Synthesis Kit
(1)
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April 28, 2022
We were interested in understanding the transcriptome response of immune cells in mice to a peptide hormone. This kit is very realizable in ...
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Good PMK2 Antibody for Western Blot
(1)
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Cell Signaling Technology
April 28, 2022
I checked this antibody on fibroblast and I stimulated with TGF-b to overexpress PKM2. Our research is focused on the SMAD pathway that ...
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Bright CD56 Antibody
(1)
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BD Biosciences
April 28, 2022
We use this antibody to detect NK cells in cell suspensions for flow cytometry. It's a great antibody that gives a bright signal and is easy...
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Fast RNA Purification Kit
(1)
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Thermo Fisher Scientific
April 28, 2022
This is a fast RNA purification kit (~10min in total) that is compatible with cultured cells. We were interested in understanding the ...
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Great Catenin/pp120 IF Antibody
(1)
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BD Biosciences
April 28, 2022
Our research aims at understanding epithelial cells in human tissue. pp120 cells are usually identified as epithelial cells. In this ...
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Time Course Effect on ST2 Polyclonal Antibody
(1)
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Thermo Fisher Scientific
April 28, 2022
ST2 is a cardiac biomarker encoded by the IL1RL1 gene. ST2 sense the signal, presence and severity of cardiac remodeling and tissue fibrosis...
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Good, Usable Anti-ß-Catenin Antibody
(1)
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BD Biosciences
April 28, 2022
We used it to detect Beta-Catenin monoclonal antibody specifically binds to Beta-Catenin. ß-Catenin is a 92 kDa protein that binds to the ...
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Great BP Cloning Kit
(1)
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April 28, 2022
Great BP cloning kit that is compatible with D-Topo cloning kit from Invitrogen. This kit is generally useful for shuttling genes of ...
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Reliable Cytokine Array for Cell Culture Media
(1)
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RayBiotech
April 27, 2022
The protocol is easy to follow and operate. This kit only requires a small volume of samples and can detect a low concentration of cytokine ...
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tdTomato Very Bright & Specific for IF, IHC Needs Work
(1)
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LSBio
April 27, 2022
I use a tdTomato Reporter mouse for my cells and have used this a lot over the years! It is very bright and specific at 1:250 for ...
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Efficient FceR1 Antibody for EMSA
(1)
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April 27, 2022
FceRI is one of the high-affinity receptor for the Fc region of immunoglobulin E. It is a tetrameric receptor complex that binds Fc portion ...
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Time Course Effect of Chemoattractant on Fc Epsilon RI beta/MS4A2 Expression
(1)
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Novus Biologicals, a Bio-Techne Brand
April 27, 2022
IgE receptor Fc epsilon RI beta gene encodes the beta subunit of the high affinity family membrane-spanning 4A gene family. FCER1 ...
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Versatile Compensation Beads for Flow Cytometry
(1)
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April 27, 2022
These compensation beads are used to bind fluorescent antibodies to create single stain controls for flow cytometry.
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Excellent Antibody for Detecting Spliced Isoform of XBP1
(1)
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BioLegend
April 27, 2022
This antibody is one of the best commercially available. It's specific for the isoform spliced of XBP1 (XBP1s). However, who uses this ...
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Good SPEG Antibody for Western Blot
(1)
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Sino Biological, Inc.
April 27, 2022
Speg is striated muscle enriched protein kinase. I used skeletal muscle from Speg KO and WT to specifically target the Speg protein. This Ab...
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Great LDH-B Antibody
(1)
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Abcam
April 26, 2022
Cells were stimulated with tgf-b in starvation medium. Our research is focused on the SMAD pathway that regulates a diverse set of ...
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Good For WB, Not For PLA
(1)
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Santa Cruz Biotechnology, Inc.
April 26, 2022
Antibody is fine for WB using human cells (iPSC-ECs and astrocytes) and mouse brain tissue. The antibody did not work for proximity ligation...
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Excellent Antibody for the Detection of IRE1
(1)
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Cell Signaling Technology
April 26, 2022
We routinely used this antibody for WB. It's very specific with no background in breast cancer cell lines. We used diluted 1:1000.
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Incubator
Injector
Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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