Good Polyclonal Prox-1 Antibody

MIRC
McMaster University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-Prox1 Antibody

Catalog Number:

925201

Clone Number:

Poly19252

Info:Supplier Page View Product Specs

Browse Similar Products: PROX1 Antibodies

I used this antibody to detect lymphatics in mouse lung tissue. I couldn't use a LYVE-1 antibody for this application since all blood vessels in mouse lung are LYVE-1+. The antibody worked reasonably well with some background staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lung

Primary Incubation

1:100 overnight @4C

Blocking Agent

Goat serum

Secondary Incubation

1:1000 1hr @ RT

Tertiary Incubation

N/A

Detection

Fluorescence microscope

Results Summary

The antibody stained lymphatic-like structures without staining blood vessels. There was some background staining, but not excessive.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Decent staining.

The Bad

Some background.

The Bottom Line

Relatively inexpensive, worth trying.

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