Good TFAM Antibody for Western Blot

June 14, 2022

BlueskyReddit
NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

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Quality of Results

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Company:

Cell Signaling

Product Name:

TFAM (D5C8) Rabbit mAb

Catalog Number:

8076

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Cells were seeded and left overnight at 37 °C and 5% CO2. The day after were stimulated for 24 hours with 0.5% FBS. I harvested with lysis buffer and protease inhibitor cocktail 1:100 (v/v). The antibody was diluted 1:1000 with 5% BSA in TBST.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Cells were seeded and left overnight at 37 °C and 5% CO2. The day after were stimulated for 24 hours with 0.5% FBS. I harvested with lysis buffer and protease inhibitor cocktail 1:100 (v/v). The antibody was diluted 1:1000 with 5% BSA in TBST.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Quality and price are extremely good.

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