Anti-IBA1 for Staining Microglia in Murine Brain Tissue

June 24, 2022

Albert Einstein College of Medicine

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Ease-of-Optimization

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Company:

Fujifilm

Product Name:

Anti-IBA1 Polyclonal Antibody

Catalog Number:

019-19741

Clone Number:

Polyclonal

Info:Supplier Page View Product Specs

Browse Similar Products: IBA1 Antibodies

Fujifilm's anti-IBA1 polyclonal antibody is one of the best reagents used in our lab for staining of murine brain tissue sections. We use it for staining of microglia, and the reagent is easy to optimize/use and further provides high quality resolution of the target cell.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine brain tissue sections

Primary Incubation

1:500; overnight @ 4 degrees C

Blocking Agent

Donkey serum or BSA

Secondary Incubation

1:500; 1 hour @ RT.

Tertiary Incubation

N/A

Detection

Fluorescent or confocal microscopy.

Results Summary

We obtain high quality, resolved microglial imaging, including both the cell body and branching structures, using this product via both fluorescent and confocal platforms.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High resolution of all microglial components; Can be used with various different imaging platforms.

The Bad

Staining deteriorates after 1-2 months time.

The Bottom Line

We highly recommend this product for use as described here. This is truly a great product!

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