Great pAMPKa Antibody for Western Blot

June 14, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-AMPKα (Thr172) (40H9) Rabbit mAb

Catalog Number:

2535

Clone Number:

N/A

Info:Supplier Page View Product Specs

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I seed the cells and I wait 24 hours leaving them at 37 °C, 5% CO2. I stimulated the cells for 24 hours and I harvested with lysis buffer and protease inhibitor cocktail 1:100 (v/v). The antibody was diluted 1:1000 with 5%BSA in TBST.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I seed the cells and I wait 24 hours leaving them at 37 °C, 5% CO2. I stimulated the cells for 24 hours and I harvested with lysis buffer and protease inhibitor cocktail 1:100 (v/v). The antibody was diluted 1:1000 with 5%BSA in TBST.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Price and quality are good.

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