Robust Bcl-xL (54H6) mAb for Western Blot

June 27, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Bcl-xL (54H6) mAb

Catalog Number:

2764

Clone Number:

54H6

Info:Supplier Page View Product Specs

Browse Similar Products: Bcl-xL Antibodies

The BCL-2 family proteins have multiple functions; among these, BCL-XL is known to inhibit apoptosis. This antibody was used to detect BCL-XL levels in cell lysates through western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:1000, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-rabbit IgG HRP (#HAF008, R&D systems), 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

Protein loading: 25 ug/mL. The antibody is consistent and provides bands at 30 kDa. Medium to high exposures is needed for detection. It can be reused at least twice when diluted in BSA/TBS.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Consistent, robust.

The Bad

Medium to high exposures for detection.

The Bottom Line

Good antibody for western blot analysis.

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