Mounting Tissue Samples with ProLong Gold Antifade Reagent

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

ProLong Gold antifade reagent

Catalog Number:

P36935

Before downstream imaging, we use ProLong Gold antifade mountant on our murine brain tissue samples. The product allows for the visualization of clear, defined cellular nuclei, but after ~2 weeks, the signal is diffuse (no longer defined) throughout the sample/background.

Experimental Design and Results Summary

Application

Imaging

Starting Material

Tissue or cell sections/mounts.

Tips

Read tissue mounts immediately; Do not mount, store, and read at a later date.

Results Summary

When sections are read imaged soon after application of the reagent, the cellular nuclei staining appears clear/well-defined and easy to assess.

DOI or PMID #

N/A

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Summary

The Good

Easy to use; Cost effective; Reliable reagent.

The Bad

Not for long term imaging projects.

The Bottom Line

Overall we recommend this product for use as described here.

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