Cell Signaling
Phospho-Y705 STAT3 Antibody
9145S
D3A7
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We knocked out an upstream JAK protein in the Cal27 cell line and wanted to examine downstream STAT3 signaling. We blotted JAK KO (KO) and control cell lines for Phospho-Y705 STAT3 in starvation and nutrient-rich cell culture conditions.
Western Blot
Cal27
1:1000 dilution of primary antibody in 1% BSA incubated overnight at 4 degrees celsius on a slow rocker.
5% milk
1:1000 goat-anti-rabbit HRP conjugated antibody suspended in 1% milk for one hour at room temperature on a slow rocker.
N/A
Film
We found that the JAK KO cells had a decrease in Phospho-Y705 STAT3 signalling both in starvation and nutrient rich conditions.
Robust bands in expected size range.
There is some limited background signal in the expected size range.
This antibody clearly detects Phos-Y705 STAT3 in the expected size range with limited background.