Fixable Viability Dye

McMaster University
MIRC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Fixable Viability Dye eFluor™ 780

Catalog Number:

65-0865-18

I use this dye in flow cytometry experiments to assess viability in samples that will be fixed later on. It works well to stain dead cells and holds up well to fixation.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Expanded NK cells and cancer cells.

Tips

Needs to be diluted in PBS before use.

Results Summary

This dye works well to stain dead cells, although it may stain more cells than a DNA binding stain would. If the samples need to be fixed, this will hold up.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Works after fixation.

The Bad

Some over-staining.

The Bottom Line

Good product, useful when fixation is necessary.

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