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Date Published
Bright Human CD45 Antibody
(1)
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BD Biosciences
June 14, 2022
I use this antibody from BD Biosciences in routine flow cytometry experiments. The antibody gives a good bright signal with little ...
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Great CD86 Antibody for Flow Cytometry
(1)
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BD Biosciences
June 14, 2022
I use this antibody frequently to stain immune cells from mouse lymph nodes for flow cytometry. It works great and the staining is strong.
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High Throughput Sample Processing with Sigma Centrifuge 4-16S
(1)
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QIAGEN - Sample to Insight
June 13, 2022
We purchased the Sigma Centrifuge 4-16S to perform high throughput sample processing for DNA and/or RNA extraction using QIAGEN's 96-well ...
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Excellent Anti-SIGLEC H Antibody
(1)
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Abcam
June 13, 2022
The antibody works well in western both at 1:500 and 1:1000 dilution depending on the protein expression in the sample. Very good antibody ...
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Transferrin Receptor Monoclonal Antibody
(1)
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Thermo Fisher Scientific
June 13, 2022
Transferrin Receptor (CD71) antibody was used for western blot analysis of HBMECs lysates. Although there is more than one band, the ...
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Western Blot: Purified Mouse Anti-p21
(1)
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BD Biosciences
June 13, 2022
This antibody was used to detect p21 expression from cell lysates. Western Blot analysis was conducted with 25 ug/mL of protein. Easy to ...
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Imaging of Light Microscopy Using Leica's Flexacam C3 Camera
(1)
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Leica Microsystems
June 13, 2022
We purchased the Flexacam C3 Camera and accompanying software to use with our existing Leica DM 1000 light microscope with the intention of ...
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Great Anti-Lamin B1 Antibody for Western Blot
(1)
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Abcam
June 13, 2022
This antibody was used to detect Lamin B1, a nuclear envelope marker, in western blot analysis. Protein loading: 25 ug/mL. Sensitive ...
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Good Puromycin Antibody for Western Blot
(1)
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June 13, 2022
A mouse model with I4895T mutation in type 1 ryanodine receptor displays myopathy including muscle weakness and atrophy. In vivo protein ...
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Simple Bench Top Light Microscope for Field Applications
(1)
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June 13, 2022
We purchased this microscope for use at our remote field sites in India. The scope is a great bang-for-buck option. Specifically, it is ...
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C/EBP ß Antibody (H-7) for Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
June 13, 2022
This antibody was used to detect C/EBP/ß expression (also known as L6DBP) from human cell lysates in western blot analysis.
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Great Kit for cDNA Synthesis
(1)
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Bio-Rad
June 10, 2022
We utilized this kit with different RNA templates from cell lines and primary cells and with a range of 100ng to 1ug RNA templates. Great ...
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Simple and Accurate 3D Cytotoxicity Assay
(1)
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Promega
June 10, 2022
Tested on collagen type I based sponge scaffold culture of ovarian cancer and fibroblasts. Used as an assessment of cell proliferation on a ...
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Great TGFb1 for Fibroblasts Activation
(1)
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Bio-Techne
June 10, 2022
We used this protein to activate fibroblasts and studying fibrosis and desmoplasia. We used a concentration ranging from 25 to 50ng/ml on ...
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Great Mini-Spin and Mixer for HUMAN’s Loopamp Workflow
(1)
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June 10, 2022
HUMAN's HuMax ITA was used in the Loopamp workflow to ensure proper, complete, and uniform mixing of all samples with the dried reagents ...
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Good Ubiquitin Antibody for Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
June 10, 2022
A mouse model with I4895T mutation in type1 ryanodine receptor displays muscle weakness and atrophy. Muscle of this mutant model showed ...
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DNA Amplification via LAMP Using HUMAN's Loopamp MALARIA Pf Detection Kit
(1)
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June 10, 2022
HUMAN's Loopamp MALARIA Pf Detection Kit was used to amplify parasite DNA via LAMP from otherwise unprocessed human whole blood samples. The...
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Bright F-Actin Staining
(1)
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Thermo Fisher Scientific
June 10, 2022
Staining of ovarian cancer cell lines on 3D sponge scaffolds collagen type I based model to detect cells' shape and penetration in the ...
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Bright CB2 Immunostaining
(1)
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NSJ Bioreagents
June 09, 2022
We optimized an immunofluorescence-based protocol for CB2 immunolocalization, getting nice staining in the whole live epidermis of the skin....
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Excellent Signal From Secondary Antibody
(1)
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Bio-Rad
June 09, 2022
I used this antibody in WB after diluting 1:5000 in NDFM 5% in TBST for 2 hours. The antibody worked perfectly, giving well-shaped and clean...
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All-In-One LAMP System
(1)
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June 09, 2022
We employed the HumaLoop M all-in-one LAMP system to amplify Plasmodium DNA from small volumes of human blood. Easy/self-installation, easy ...
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Excellent User-Friendly Sorter for Simple Experiments
(1)
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Sony Electronics, Sony Corporation
June 09, 2022
We are interested in utilizing TCRs for cancer immunotherapy. Consequently, we are sorting naive T-cells or modified cell lines for the ...
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Excellent Caspase-3 Antibody
(1)
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Cell Signaling Technology
June 09, 2022
I used this antibody for WB on SHSY5Y studying apoptosis after amyloid beta treatment. I diluted 1:1000 in BSA 5% in TBST 1x and incubated O...
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Good Western Blot Antibody for TYK2 Protein
(1)
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Cell Signaling Technology
June 09, 2022
We generated a CRISPR-Cas9 Knockout of the TYK2 gene and wanted to determine whether the knockout was validated on the protein level. We ...
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HUMAN's Loopamp PURE DNA Extraction Kit Great for DNA Extraction From Human Samples
(1)
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June 09, 2022
HUMAN's Loopamp PURE DNA Extraction Kit was used to extract parasite DNA from otherwise unprocessed human whole blood samples. Only a small ...
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Great Serum Haptoglobin ELISA Kit
(1)
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Aviva Systems Biology
June 08, 2022
We are interested in the roles of a class of unknown small peptides. Preliminary data suggest that these small peptides may enhance the ...
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High Sugar Diet for NASH Mice Model
(1)
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Research Diets Inc.
June 08, 2022
We are interested in studying the potential functions of a novel class of small peptides. Preliminary data suggest certain peptides may ...
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Good CD127 for T-Reg Cells
(1)
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BD Biosciences
June 08, 2022
Regulatory T cells (T-reg) have become central in the follow-up of patients whose immune system is disturbed. To identify T-reg cells, CD127...
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Great Leptin ELISA Kit
(1)
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June 08, 2022
We are interested in a new class of small peptides in circulation. Preliminary data suggest they may have roles in body weight control. We ...
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Great NCAM-1 for Western Blot
(1)
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R&D Systems, a Bio-Techne Brand
June 08, 2022
Human pluripotent stem cells (hPSCs) were cultured in a pluripotent maintenance medium. NCAM1 (a known neural-restricted protein involved ...
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Great PDGF Used for Western Blot Detection
(1)
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Cell Signaling Technology
June 08, 2022
I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and treated them ...
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Great CD22 Antibody for B Cell Identification
(1)
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June 08, 2022
Typically, B cells are characterized by their positive expression of CD19 or CD20. However, with modern antibody therapy, e.g. Rituximab, ...
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Great Bcl-xL Detection With WB
(1)
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Cell Signaling Technology
June 08, 2022
I treated the epithelial cells with eiF4E siRNA for 24 hours. The knockdown of eIF4E suppresses cell growth and correlates with increase in ...
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Excellent mTOR Antibody
(1)
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Cell Signaling Technology
June 07, 2022
I used mTOR antibody for studying proliferation of neuronal cells SHSY5Y after treatment of amyloid beta by WB. Antibody worked perfectly ...
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pAKT Western Blot Detection
(1)
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Cell Signaling Technology
June 07, 2022
I used this antibody on epithelial cells starved for 4 hours (0%FBS) and treated with eIF4E siRNA for 24 hours with F-12k medium (2% FBS). ...
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Bright Fibrinogen Staining on Human Platelets
(1)
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Agilent Technologies
June 07, 2022
Polyclonal Rabbit Anti-Human Fibrinogen/FITC allows the detection of fibrinogen that is bound to the platelet surface. Bright staining of ...
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Great Growth Hormone ELISA Kit
(1)
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MilliporeSigma
June 07, 2022
We are interested in studying the roles of novel peptide hormones in mice. One of the potential functions is the stimulation of growth ...
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Great Cleaved Caspase-8 for Western Blot
(1)
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Cell Signaling Technology
June 07, 2022
Epithelial cells were seeded. After 24 hours I used starvation medium (0% FBS) for 4 hours and I treated with eiF4E siRNA for 24 hours with ...
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Excellent Bax Antibody From CST
(1)
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Cell Signaling Technology
June 07, 2022
I used this antibody on SHSY5Y, MRC5 and A549 cells studying cell proliferation and apoptosis. Antibody was diluted 1:1000 in BSA 5% and ...
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Caspase-3 Western Blot Detection
(1)
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Cell Signaling Technology
June 07, 2022
I used this antibody on epithelial cells starved for 4 hours (0% FBS) and treated with eIF4E siRNA for 24 hours with F-12k medium (2% FBS). ...
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Bright C3/C3b/iC3b Staining on Platelets
(1)
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BD Biosciences
June 07, 2022
BV750 Mouse Anti-C3/C3b/iC3b recognizes the components and fragments of complement C3, which may be present on the platelet surface in ...
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Very Reliable PAX6 Antibody
(1)
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BD Biosciences
June 06, 2022
I apply large-scale multi-omics assays in human samples for basic and translational biomedical research, from scRNA-Seq to epigenomics and ...
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NADP/NADPH Assay Kit Worked Well
(1)
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Abcam
June 06, 2022
The goal of this research is to evaluate NADP, NADPH in mouse derived macrophages. We seed cells in 96-well plate and used this kit to ...
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Great Anti-SOD2/MnSOD Antibody
(1)
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Abcam
June 06, 2022
The background of this research is to check cell proliferation in rat pulmonary derived smooth muscle cells (SMC). We used anti-SOD2/MnSOD ...
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NuPAGE™ 4 to 12%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels
(1)
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June 06, 2022
These precast polyacrylamide gels were used for electrophoresis (western blot protocol). Gels provide "broad molecular weight protein ...
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Great Dye for Live Cell Imaging
(1)
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June 06, 2022
This dye from Thermo Fisher Scientific was tested on fibroblasts and ovarian cancer cell lines. It worked on both 2D and 3D sponge scaffolds...
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Great Kit for Fast Isolation of Exosomes
(1)
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June 06, 2022
Exosomes can be reliably isolated from ultracentrifuged cell culture media. Small contamination from microvesicles, but can be easily ...
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Reliable Compensation Beads for Flow Cytometry
(1)
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June 06, 2022
I used these beads when I have multi-color antibodies for flow cytometry experiments. I have been using these beads for more than 6 years. ...
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Best Anti-Lamin B2 Antibody That I've Used
(1)
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BioLegend
June 06, 2022
I performed indirect immunofluorescent staining in PFA-fixed and MeOH-fixed mouse embryonic fibroblasts (MEFs). The wild-type cells stained ...
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Good QC Fluorospheres for FACS
(1)
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June 03, 2022
We are using CytoFLEX Flow Cytometer for cell sorting and cell cycle. This machine has two types of sample loading; plate and tube. We ...
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Camera
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Western Blot
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Cell Culture
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qPCR
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Concentration
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Mutagenesis
FISH
CRISPR
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In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
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Human
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Arabidopsis
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Virus
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Drosophila
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Tissue
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Adipose
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In Vivo
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Peripheral Blood
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Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
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Induced Pluripotent Stem Cell
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Macrophage
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Microvascular Endothelial Cell
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PBMC
Platelets
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Thymocytes
Tumor Cells
Cell Line
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HeLa
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U2OS
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HL60 (HL-60)
A375 (A-375)
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MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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