Sigma Aldrich
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
21888
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I use this reagent for staining cells in a cytotoxicity assay. Cells are easily stained with this dye non-discriminately, and it gives a good signal even after fixation. This is a good dye for marking all cells.
Flow cytometry
Cancer cells
The dye may be toxic to some cells.
The dye stained the cells well and could be detected easily after fixation. Using higher concentrations of the dye will yield a brighter signal that can be used for proliferation assays.
N/A
Good staining.
Some background.
A good dye for marking all cells.