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Date Published
Good CD45 Antibody
(1)
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BioLegend
June 03, 2022
We wanted to phenotype immune cells in a mouse tumor, so we used CD45 to identify mouse immune cells. The antibody stains CD45 very clearly,...
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Great Gel for Separating Small MW Proteins
(1)
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June 03, 2022
We are interested in small peptide hormones regulating immune system. The experiment using this Tricine gel is to detect small molecular ...
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Excellent Staining with hCD3 Antibody
(1)
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BioLegend
June 03, 2022
We were phenotyping some human PBMCs, so we used hCD3 to pull out human T cells from other human immune cell populations. The antibody ...
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PrestoBlue Cell Viability Reagent
(1)
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June 03, 2022
PrestoBlue was used to measure cell viability of human endothelial cells upon exposure to a certain drug. Results (absorbance levels) were ...
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Good Staining with hIFNg Antibody
(1)
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BD Biosciences
June 03, 2022
We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hIFNg to assess cytokine secretion following in vitro ...
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Good Antibody for 2D and 3D Staining
(1)
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Abcam
June 03, 2022
We tested this antibody on both 2D cell lines and 3D cultured cell lines (mrc5, huvec) at a concentration ranging from 1:50 to 1:250 ...
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Great Staining with hCD69 Antibody
(1)
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BioLegend
June 02, 2022
We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hCD69 to pull out and analyze more activated human T ...
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High Sensitivity Detection with PARP Antibody
(1)
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Cell Signaling Technology
June 02, 2022
I used PARP (46D11) Rabbit mAb for Western Blot at the suggested dilution (1:1000) in TBST 1X / 5% Not fat dry milk, at overnight incubation...
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Reliable Glucose Assay Kit
(1)
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Abcam
June 02, 2022
The background of this research is to measure glucose level in the mouse serum sample. We used this Glucose Assay Kit. This kit is very ...
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Reliable Transfection Kit
(1)
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June 02, 2022
Reliable transfection kit that can be used with different cell lines. The kit contains 1 vial of Lipofectamine Reagent and 1 vial of P3000TM...
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Good Mutagenesis Kit
(1)
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Agilent Technologies
June 02, 2022
The research goal is to check protein activity in normal or mutated proteins. This kit is used to generate mutation at specific locations in...
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Antibody Stains hFoxP3 Very Well
(1)
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BioLegend
June 02, 2022
We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hFoxP3 to pull out and analyze human Tregs in the mice....
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Excellent hTNFa Antibody from BioLegend
(1)
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BioLegend
June 02, 2022
We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hTNFa to assess cytokine secretion following in vitro ...
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Good Protease Inhibitor
(1)
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Thermo Fisher Scientific
June 02, 2022
We used this protease inhibitor for western blot. Each tube has 100uL and 100X dilution factor with RIPA buffer. Easy to use. Works well for...
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Excellent Precast Gels
(1)
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Bio-Rad
June 01, 2022
I've been using these gels since 2+ years and I am fully satisfied on how proteins can be separated even if MW gap is very narrow. Used at ...
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Reliable APOE Antibody
(1)
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BioLegend
June 01, 2022
This antibody was used to quantify APOE in human FFPE brain tissue. We stained with this antibody successfully in IHC, IF, and Akoya Opal. 1...
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Bright Staining with hKi67 Antibody
(1)
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BioLegend
June 01, 2022
We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hKi67 to pull out and analyze more proliferative human ...
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hCD25 Antibody Stains Very Well
(1)
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BioLegend
June 01, 2022
We were phenotyping some human PBMCs, so we used hCD25 to pull out more activated human T cells from other human immune cell populations. ...
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Excellent RIPA Buffer
(1)
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June 01, 2022
I've used this one for over 10 years. Working is really well for cells and animal tissues. After 30 min incubation in ice, fat is floating ...
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Excellent and Clean Staining
(1)
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June 01, 2022
I used this Phalloidin on MRC-5, SHSY5Y cells, A549, Mesenchymal stem cells and HUVECs. After following the instructions from the vendor, ...
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Great PKM2 Rabbit mAb Antibody from CST
(1)
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Cell Signaling Technology
June 01, 2022
Our research is focused on studying glycolysis pathways of bone marrow derived macrophages. As PKM2 is one of the key enzymes for glycolysis...
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Bright CD279 Antibody from BioLegend
(1)
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BioLegend
June 01, 2022
We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hPD-1 to pull out and analyze more activated human T ...
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Robust and Versatile Analytical Instrument
(1)
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May 31, 2022
Thermal Analysis System DSC3 is a robust and versatile analytical instrument having rugged the MultiSTAR sensor with 56 thermocouples that ...
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Bright Staining with hCD8a Antibody
(1)
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BioLegend
May 31, 2022
We were phenotyping some human PBMCs, so we used hCD8a to pull out CD8+ human T cells from other human immune cell populations. The product ...
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Bright Staining with CD4 Antibody
(1)
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BioLegend
May 31, 2022
We were phenotyping some human PBMCs, so we used hCD4 to pull out CD4+ human T cells from other human immune cell populations. The antibody ...
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Antibody Stains hCD3 Very Well
(1)
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BioLegend
May 31, 2022
We were phenotyping some human PBMCs, so we used hCD3 to pull out human T cells from other human immune cell populations. The antibody ...
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Good Ku80 Antibody for Total Protein Quantification
(1)
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Cell Signaling Technology
May 31, 2022
We knocked out a member of the JAK protein family in two head and neck cancer cell lines, and we wanted to determine if there were any ...
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Antibody Stains HLA-DR Very Well
(1)
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BioLegend
May 31, 2022
We were phenotyping some human PBMCs, so we used HLA-DR to pull out B cells and activated immune cells from other human immune cell ...
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Secondary Antibody Resulted in Cross-Reactivity with Rat
(1)
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Jackson ImmunoResearch Laboratories, Inc.
May 31, 2022
I coated cover glasses with rat anti-MHCII antibodies and seeded dendritic cells on them to see the surface expression of several receptors ...
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High Quality Mountant
(1)
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May 31, 2022
I applied ProLong Diamond Antifade Mountant with DAPI in fixed RT4 cells. We have a great picture of DAPI. Very easy to access. Great signal...
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CD80 Antibody Works Very Well for Flow Cytometry
(1)
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BioLegend
May 27, 2022
We wanted to phenotype human macrophages in an in vitro culture, so we compared hCD16 expression on human macrophages compared to monocytes....
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Modified Centrifuge Settings Are Not Maintained
(1)
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Fisher Scientific
May 27, 2022
Our lab processes human tissues to isolate lymphocytes for studies, so we do many density gradient centrifugation spins. Touch screen ...
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Antibody Stains hCD3 Very Well
(1)
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BioLegend
May 27, 2022
We were phenotyping some human PBMCs, so we used hCD3 to pull out human T cells from other human immune cell populations. This product is ...
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Wonderful Ultracentrifuge for Any Lab
(1)
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May 27, 2022
The rotors are easily loadable to allow centrifuging of exosomes and viral preps. Although a little expensive, this machine is incredibly ...
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Gravimetric Dilutions with Minimal Hands-On Time
(1)
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Agilent Technologies
May 27, 2022
The 7696A Sample Prep Workbench is used for most of the gravimetric dilutions performed in the lab. Having a dedicated weighing station (...
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Antibody Brightly Stains hCD14
(1)
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BioLegend
May 27, 2022
We wanted to phenotype human macrophages in an in vitro culture, so we compared hCD14 expression on human macrophages compared to monocytes....
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Time Consuming Kit, Low Sample Purity
(1)
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May 27, 2022
We got a sample of this kit to test using it for enrichment of memory T cells prior to FACS for T cell stimulation experiments. We were able...
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Good Staining with hCD16 Antibody
(1)
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BioLegend
May 26, 2022
We wanted to phenotype human macrophages in an in vitro culture, so we compared hCD16 expression on human macrophages compared to monocytes....
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Good Expression with AKT Antibody for Western Blot
(1)
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Cell Signaling Technology
May 26, 2022
I evaluated the total amount of AKT on A549 (epithelial lung cells) transfected for 24 hours with eIF4E siRNA. I got great results, and all ...
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Assay Kit More Time Consuming Than Expected
(1)
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May 26, 2022
We use these negative enrichment kits to enrich for T cells before FACS to reduce sorting time. The sorted cells are used for stimulation ...
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Kit Works with EasySep Magnet
(1)
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May 26, 2022
We use these T cell enrichment kits to enrich our samples before FACS to reduce our sorting times. The enriched samples are used for ...
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Great Contaminant Identification Technology
(1)
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Thermo Fisher Scientific
May 26, 2022
We extract DNA from T cells using salt and ethanol extraction methods, and the carbon content in the DNA is measured for carbon dating.
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Good Kit for Detecting Human Exosomes via Tetraspanin
(1)
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May 26, 2022
Kit can detect exosomes using standard tetraspanin markers CD9, CD63, and CD81. Note that there are multiple washing steps, and handling the...
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Effective Kit for Detecting Intra-Exosomal Cargo
(1)
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May 26, 2022
This kit worked well at detecting proteins within exosomes. Multiple wash steps are required, so timing is critical. Resulted in strong ...
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Nice Anti-Vimentin Antibody
(1)
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Abcam
May 25, 2022
I used this antibody to check expression of vimentin in A549 lung cancer cells. I did western blot and immunofluorescence staining. This ...
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Great eIF4E Antibody for Western Blot
(1)
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Cell Signaling Technology
May 25, 2022
I seeded epithelial cells (A549) and I transfected with eIF4E siRNA after 24 hours. I seeded 2 different seeding densities (180k and 200k ...
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Good Resolution with E-Cadherin Antibody, Some Residual PBMC Staining
(1)
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BD Biosciences
May 25, 2022
E-cadherin expression was studied in PBMCs, with difficult-to-believe results because of the expression patterns. But we also performed a ...
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Titration Required but Yields Pure Samples
(1)
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May 25, 2022
We isolate T cells from human tissues and extract the DNA for carbon dating analysis. These kits allow us to isolate T cells without the use...
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Antibody Stains hCD163 Very Well
(1)
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BioLegend
May 25, 2022
We wanted to phenotype human macrophages in an in vitro culture, so we used hCD163 as a differentiation marker to compare to monocytes.
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Excellent Antibody for WB
(1)
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Cell Signaling Technology
May 25, 2022
Very good antibody tested in WB. Used on PVDF membrane, primary diluted 1:1000 in 5% BSA and secondary used 1:5000 for 2 h. Bands are clean ...
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ELISA Kits
Enzymes
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Incubator
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Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
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Western Blot
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ELISA
Immunofluorescence
Immunoprecipitation
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Block/Neutralize/Inhibit
Cell Isolation
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Staining
Cell Culture
DNA Purification
PCR
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RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
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Cell Counting
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Library Construction
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Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
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Horse
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C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
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Blood
Blood Vessel
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Bone Marrow
Brain
Breast
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Cerebral Cortex
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Cornea
Coronary Artery
Dermis
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Embryo
Endometrium
Epidermis
Eye
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Hair
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In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
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Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
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Rectum
Retina
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Spinal Cord
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Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
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Glial Cell
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Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
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HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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