Robust Effect of Chemoattractant on ORAI1 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Invitrogen

Product Name:

ORAI1 Monoclonal Antibody

Catalog Number:

MA5-15776

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: ORAI1 Antibodies

Orai1 is a calcium selective ion channel that is encoded by the ORAI1 gene. Orai channels play crucial role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency in humans. Here we checked the time course effect of Orai1 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We performed western blot and detect, analyzed the Orai1 expression. We found that there is a no change in Orai1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This is a antibody and works great for western blot.

The Bad

None

The Bottom Line

This antibody works great for western blot and microscopy. Highly recommended.

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