Actin Antibody Works Great

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Anti-Actin Antibody, clone C4

Catalog Number:

MAD1501

Clone Number:

N/A

Info:Supplier Page 

Browse Similar Products: ACTIN Antibodies

I used this antibody for Western blot on protein extracted from human cortical organoids and it worked perfectly! The bands came up at the correct size with very strong expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cortical Organoids

Primary Incubation

1:1000 overnight at 4C

Blocking Agent

5% milk

Secondary Incubation

1:1000 Goat anti Mouse IgG (H+L) Secondary Antibody, HRP for 1 hour at RT

Tertiary Incubation

N/A

Detection

Pierce™ SuperSignal™ West Pico PLUS Chemiluminescent

Results Summary

Both samples used stained for actin. The bands came up at the correct size with very strong expression.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works great.

The Bad

Expensive.

The Bottom Line

Expensive but great, and I would highly recommend using this for Western blot (human samples).

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