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Date Published
Good CD335 (NKp46) Antibody
(1)
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BioLegend
April 24, 2015
We selected NKp46 Antibody from Biolegend specifically because NKp46 is expressed in NK cells and can be found in lymph nodes. Using this ...
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Tryple- Alternative to trypsin
(1)
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Thermo Fisher Scientific
April 24, 2015
This product can be used for very fast dissociation of hard to trypsinize cells without the need to inactivate with FBS or SBTI. The tryple ...
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It extracts DNA but it is not pure
(1)
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QIAGEN - Sample to Insight
April 24, 2015
I used this Gel DNA recovery kit to extract DNA from agarose fragments that were subsequently used for cloning purposes.
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FITC anti-mouse CD11c Antibody for Dendritic Cell Staining
(1)
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BioLegend
April 24, 2015
Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, and stained with FITC anti-mouse CD11c Antibody.
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Makes marking band sizes easy
(1)
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April 23, 2015
This was used as the ladder when running Western blots to mark molecular weights on the gel. Used with 4-15% gels and transferred to PVDF ...
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Very Good Anti-Human CD45 antibody
(1)
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BD Biosciences
April 23, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this ...
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Good and useful reagent
(1)
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April 23, 2015
The aim of this research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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Very good antibody for detecting human Cd4 expression
(1)
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April 23, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this ...
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Good pan-JAK Inhibitor
(1)
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Cayman Chemical
April 22, 2015
I use this compound to inhibit JAK/STAT signaling in my cells. This compound effectively inhibits IL-13 activation of the JAK/STAT pathway, ...
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Good for Duplex PCR
(1)
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Bio-Rad
April 22, 2015
I use this supermix when performing duplex Taqman probe PCR. It amplifies both probes and saves time and sample because I can do duplexing ...
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Very Good Anti-Human CD45 antibody
(1)
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April 22, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. This antibody was ...
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Very Good Anti-Human CD3 antibody
(1)
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April 22, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions prepared from spleen were used for...
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Purchasing through Quartzy
(1)
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April 21, 2015
Purchasing through Quartzy can potentially save lots of money. It gives you the advantage of potentially finding better prices than the ones...
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Good for Sybr Green PCR
(1)
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Bio-Rad
April 21, 2015
I was using this to perform quantitative PCR on a cDNA sample or a no template control. The cDNA sample showed amplification but the no ...
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Good phospho STAT6 Antibody
(1)
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Cell Signaling Technology
April 21, 2015
I was using the phospho-STAT6 antibody to determine if the IL-13 I purchased was causing activation of the JAK/STAT pathway in primary ...
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Very good antibody for detecting human Cd45 expression
(1)
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April 21, 2015
I have used this antibody to separate human C45+ cells. The staining looks good with two separate populations of CD45 positive and negative ...
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ROR levels in human lung tissue
(1)
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Abcam
April 20, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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APC anti-mouse CD11c Antibody for Dendritic Cell Staining
(1)
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BioLegend
April 20, 2015
Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, and stained with APC anti-mouse CD11c Antibody. I used CD11c ...
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Ku70 antibody for mouse lung samples
(1)
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Abcam
April 20, 2015
We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific ...
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Immunofluorescence staining using Life Technologies AF 2nd Ab
(1)
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Thermo Fisher Scientific
April 20, 2015
We do a lot of confocal microscopy in our laboratory. Life technologies is one of our favorite companies to get 2nd antibodies from.
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Very Good Anti-Human CD4 antibody
(1)
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April 17, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions prepared from the spleens were ...
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Sox11 from US Biological - works well for mouse retina
(1)
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United States Biological
April 17, 2015
This antibody was used in a study investigating the role of transcription factor Sox11 in the adult mouse retina. Several antibodies were ...
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
(1)
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Abcam
April 17, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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Very good Bmal1 antibody for immunoblotting
(1)
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Abcam
April 16, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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Easy RNA clean up
(1)
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QIAGEN - Sample to Insight
April 16, 2015
Used this kit to clean up RNA from several sources: tissue, primary neurons, cell lines. The end goal is to run qPCR on these samples and ...
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Good HIF-2alpha antibody
(1)
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April 16, 2015
I was testing to determine if hypoxia increased HIF-2alpha (EPAS1) levels in primary human epithelial cells. I used this antibody to detect ...
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Smoke Robot
(1)
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April 16, 2015
We study the effects of cigarette smoke in airway epithelial cells. This is a great model to expose the cells to whole cigarette smoke (...
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DENV-2 Immunostaining with Anti -dengue Type 2
(1)
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United States Biological
April 15, 2015
Dengue virus (DENV-2) infected HeLa cells were immunostained for P- bodies and DENV-2. Cells grown on cover slips were washed in PBS, fixed ...
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Cotinine ELISA for mouse
(1)
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April 15, 2015
We measure cotinine levels in mouse plasma samples immediatley after exposure to E-cigarettes for 3 days (5Hr/day). Samples between control ...
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Good secondary antibody
(1)
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Thermo Fisher Scientific
April 15, 2015
This is a good secondary antibody that detects primary mouse antibodies and gives strong signal for fluorescent microscopy.
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Cost effective and easy
(1)
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Thermo Fisher Scientific
April 15, 2015
Used this kit to convert to cDNA from primary neurons, cell lines, and DNA from tissues with the ultimate goal of running qPCR on the ...
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Good source of IL-13
(1)
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April 14, 2015
Used this IL-13 to activate JAK/STAT signaling in my cells. IL-13 increased STAT6 phosphorylation, which was my assay for activation of JAK/...
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EPAC1 Western blot
(1)
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Cell Signaling Technology
April 14, 2015
Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in NHBE ...
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Reliable CD45 antibody
(1)
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April 14, 2015
We were trying to distinguish microglia and astrocytes, by flow cytometry, from mixed glial cultures cultured for 4-6 weeks from pup brains....
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Good antibody for detecting Lamin A/C
(1)
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Santa Cruz Biotechnology, Inc.
April 14, 2015
I used this antibody to detect Lamin A/C in cytoplasmic (cyto.) and nuclear (nucl.) fractionated samples to assay the level of contamination...
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Good antibody to detect Notch1
(1)
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Abcam
April 13, 2015
Used this antibody to detect Notch1 protein from whole cell lysates. Antibody recognized a single band at the molecular weight shown from ...
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Good DNA Ladder
(1)
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New England Biolabs
April 13, 2015
This is the go to DNA ladder in our lab when we want to look at sizes between 100 bp and 10,000 bp. It is easy to mix and load onto the gel ...
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Very good p16 antibody for immunoblotting
(1)
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Abcam
April 13, 2015
We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific ...
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MUC5AC Immunfluorescence Staining in NHBE
(1)
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Santa Cruz Biotechnology, Inc.
April 13, 2015
This antibody was purchased to use as a marker of goblet cells in airway epithelial cells.
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NR1D1 Antibody used for Western blotting
(1)
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Novus Biologicals, a Bio-Techne Brand
April 10, 2015
We are evaluating changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological ...
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NE-PER Nuclear and cytoplasmic extraction reagent
(1)
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April 10, 2015
This was used to isolate the nuclear extract from the cell lines in order to check the nuclear protein activity in compound treated cells. ...
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Good IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate
(1)
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Thermo Fisher Scientific
April 10, 2015
Primary mouse antibody against acetylated tubulin was detected. It gives a strong signal easily detectable in confocal microscopy and low ...
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E-cadherin / CDH1 Antibody Immunofluorescence
(1)
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Thermo Fisher Scientific
April 10, 2015
Cadherin formation is one of the steps among many in the process of airway epithelial cell differentiation. In our laboratory we use this ...
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Hi Fidelity Polymerase
(1)
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New England Biolabs
April 09, 2015
I used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. ...
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Very good HA antibody
(1)
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Cell Signaling Technology
April 09, 2015
I used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear...
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Super signal west dura extended duration substrate
(1)
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April 09, 2015
This product was used to check the differential protein expression in proliferation and differentiation of neuronal cells involved in ...
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ZO-1/TJP1 Immunofluorescence
(1)
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Thermo Fisher Scientific
April 09, 2015
In our laboratory we have to track airway epithelial cells throughout differentiation. Tight junction formation is among those steps.
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Secondary Ab donkey anti Rabbit AF555
(1)
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Thermo Fisher Scientific
April 08, 2015
Secondary antibodies from Thermo Fisher Scientific are great for doing immunofluorescence. We work with primary cells (airway epithelial ...
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Ready to use kit for glucose
(1)
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Cayman Chemical
April 08, 2015
I am looking at the effect of eating yogurt before a meal on postprandial blood glucose change. Due to the large quantity of my samples, I ...
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Easy to use for protein quantification
(1)
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April 08, 2015
This product was used in Western blot assays looking at various proteins in the brain homogenate of MPTP-intoxicated animals. After ...
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