Thermo Scientific Pierce
SuperSignal West Pico Chemiluminescent Substrate
34080
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This product was used in Western blot assays looking at various proteins in the brain homogenate of MPTP-intoxicated animals. After isolating the region of interest, protein was purified, run on a gel, and transferred to a PVDF membrane. Membrane was developed with equal parts of SuperSignal reagents for 5 minutes and imaged.
Western blot
Brain homogenate
Purify protein before running on a gel and transferring to a PVDF membrane. Mix equal parts of SuperSignal reagents just prior to use (approx 2.5 mls each per membrane) and incubate at RT for 5 minutes.
May not work well to develop membranes incubated with weak primary antibodies or if the protein of interest is not well expressed.
Used with beta actin primary antibodies, this works great
None
Easy to use
May not work for all antibodies
When we need to quantify protein and normalize to beta actin, this is what we use.
Product Name: SuperSignal West Pico Chemiluminescent SubstrateSupplier Page