NE-PER Nuclear and cytoplasmic extraction reagent

April 10, 2015

indian institute of chemical technology
chemical biology

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Company:

Thermo Scientific

Product Name:

NE-PER Nuclear and cytoplasmic extraction reagents

Catalog Number:

78833

This was used to isolate the nuclear extract from the cell lines in order to check the nuclear protein activity in compound treated cells. It was chosen for its ease of isolation and because it is less time consuming.

Experimental Design and Results Summary

Application

To isolate the nuclear proteins

Starting Material

Cancer cell line

Protocol Overview

Protocol is followed as per the user guide of the supplier. For more nuclear extract, increase initial cell number. To get more concentrated nuclear extract dissolve it in less (2-4 fold) NER solution. All the steps should be done at 4 degrees C.

Tips

The reagent volume (CER-I, CER-II, NER) needs to be added as per the number of cells used for isolating the nuclear and cytoplamic extract. 

Results Summary

The cytoplasmic and nuclear extracts separated from the cancer cell line.

Additional Notes

None

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Summary

The Good

Less complexity of reagent preparation, less time consumption, high cytoplamic and nuclear extraction yield.

The Bad

More time consumption for nuclear extract compare to cytoplasmic extract.

The Bottom Line

Good product for separating the nuclear and cytoplasmic extract with high yield in less time.

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